SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKRE6
Systematic nameYPR159W
AliasCWH48
DescriptionBeta-glucan synthesis-associated protein KRE6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32486
SGD YPR159W
Gene Ontology (GO)
Cellular componentGO:0000137Golgi cis cisternaIDA:SGD.
Cellular componentGO:0016021integral to membraneIDA:SGD.
Molecular functionGO:0015926glucosidase activityIMP:SGD.
Biological processGO:00060781,6-beta-glucan biosynthetic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
116Phosphoserine.
134Phosphoserine.

Sequence Information
Sequence length720
Molecular weight80123

	---------+----------+----------+----------+----------+
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG   50
LTNPFMGSDE ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV   100
SDYKGYYSRN NSRAVSTAND NSFLQPPHRA IASSPSLNSN LSKNDILSPP   150
EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG NESSASFSSN PFLGEQDFSP   200
FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID DFKYMDKRSA   250
SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS   300
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT   350
APDVHYDATK DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN   400
KVCFTQGALE ISANLPNYGR VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS   450
YESCDAGITP NQSSPDGISY LPGQKLSICT CDGEDHPNQG VGRGAPEIDV   500
LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT TTTMNTYAGG   550
PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP   600
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV   650
MSIDYVRIYQ PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY   700
TFPKNILTGK CTSSKFKLSS                                     720