SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKNS1
Systematic nameYLL019C
DescriptionDual specificity protein kinase KNS1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32350
SGD YLL019C
Gene Ontology (GO)
Molecular functionGO:0004674protein serine/threonine kinase activityIDA:SGD.
Molecular functionGO:0004713protein-tyrosine kinase activityIDA:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.12.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
277Phosphoserine.

Sequence Information
Sequence length737
Molecular weight83843

	---------+----------+----------+----------+----------+
MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE   50
MFARQNSFLT DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR   100
IINTTINNSN NHNSSRVDED ADDDIIFIKE QPIQFSSPLI LPSSSSINNN   150
NNIVTSNNPG CGTAATSNST YITTPKKFKK QRTISLPQLP LSKLSYQSNY   200
FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL TIAHSTSPFS   250
NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV   300
YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR   350
YREAAKTELR ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI   400
YDFMCSNGIA RFPGSHIQAI ARQLIRSVCF LHDLGIIHTD LKPENILICD   450
ETHIAQKLPL KTVQSLSKRR REASKGKRKI LKNPEIKIID FGSAIFHYEY   500
HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG ESLYPIHENL   550
EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL   600
SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW   650
NLPPEKNWSL INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM   700
FEFDPTKRIT AKDALDHEWF NLGILDDGIA TYNNTQG                 737