SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKKQ8
Systematic nameYKL168C
DescriptionProbable serine/threonine-protein kinase KKQ8

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36004
SGD YKL168C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
29Phosphoserine.
31Phosphoserine.
242Phosphoserine.
248Phosphoserine.
251Phosphoserine.

Sequence Information
Sequence length734
Molecular weight83656

	---------+----------+----------+----------+----------+
MMDSKTLTAS MVMQEEKKRQ QPVTRRVRSF SESFKNLFRP PRSRDSSPIN   50
VTRIPYRSSS TSPKRSSEPP RRSTVSAQIL DPKNSPIRQR SYTLKCCTPG   100
LSHPFRQTGS GASNSPTRHR SISGEEQEIV NSLPEYKRSA SHTFHGIRRP   150
RSRSSSVSSC DSSNGTTSSS DSQWAMDSLL DDSDNDLTPY RGSNKDILKS   200
KDRAPYNYID DYNKKALRRA TSYPNPLPSK QFYNERLYTR RSHPDEESLE   250
SLPRFAGADV QCIIEQNGFK VYEDGSHEHN IKLSGVIAKL EKGNSLPAHR   300
QGSLSRPRLG ITLSGLFKHH KNECDIENAL SLLPNVEKSQ TNHEKRTGQS   350
PNDSNRSSPT QGREDYLKIV NPDASLGSDE LKLINSLSSR IHKSLQNYLQ   400
EKNLKPAECI GEQAPTFQDN YGHPVGLVGA GAYGEVKLCA RLRNEKDSPP   450
FETYHDSKYI YYAVKELKPK PDSDLEKFCT KITSEFIIGH SLSHYHKNGK   500
KPAPNILNVF DILEDSSSFI EVMEFCPAGD LYGMLVGKSK LKGRLHPLEA   550
DCFMKQLLHG VKFMHDHGIA HCDLKPENIL FYPHGLLKIC DFGTSSVFQT   600
AWERRVHAQK GIIGSEPYVA PEEFVDGEYY DPRLIDCWSC GVVYITMILG   650
HYLWKVASRE KDMSYDEFYK EMQRKNQFRV FEELKHVNSE LATNRKIALY   700
RIFQWEPRKR ISVGKLLDMQ WMKSTNCCLI YDST                    734