SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKIP1
Systematic nameYBL063W
AliasCIN9
DescriptionKinesin-like protein KIP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDC20   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p."
Gordon DM, Roof DM.
Proc Natl Acad Sci U S A. 98(22):12515-20 (2001) [11606759]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP28742
SGD YBL063W
Gene Ontology (GO)
Cellular componentGO:0005871kinesin complexTAS:SGD.
Cellular componentGO:0005876spindle microtubuleTAS:SGD.
Cellular componentGO:0005816spindle pole bodyIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0003777microtubule motor activityTAS:SGD.
Molecular functionGO:0005200structural constituent of cytoskeletonIPI:SGD.
Biological processGO:0007020microtubule nucleationIPI:SGD.
Biological processGO:0000092mitotic anaphase BIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1111
Molecular weight125795

	---------+----------+----------+----------+----------+
MARSSLPNRR TAQFEANKRR TIAHAPSPSL SNGMHTLTPP TCNNGAATSD   50
SNIHVYVRCR SRNKREIEEK SSVVISTLGP QGKEIILSNG SHQSYSSSKK   100
TYQFDQVFGA ESDQETVFNA TAKNYIKEML HGYNCTIFAY GQTGTGKTYT   150
MSGDINILGD VQSTDNLLLG EHAGIIPRVL VDLFKELSSL NKEYSVKISF   200
LELYNENLKD LLSDSEDDDP AVNDPKRQIR IFDNNNNNSS IMVKGMQEIF   250
INSAHEGLNL LMQGSLKRKV AATKCNDLSS RSHTVFTITT NIVEQDSKDH   300
GQNKNFVKIG KLNLVDLAGS ENINRSGAEN KRAQEAGLIN KSLLTLGRVI   350
NALVDHSNHI PYRESKLTRL LQDSLGGMTK TCIIATISPA KISMEETAST   400
LEYATRAKSI KNTPQVNQSL SKDTCLKDYI QEIEKLRNDL KNSRNKQGIF   450
ITQDQLDLYE SNSILIDEQN LKIHNLREQI KKFKENYLNQ LDINNLLQSE   500
KEKLIAIIQN FNVDFSNFYS EIQKIHHTNL ELMNEVIQQR DFSLENSQKQ   550
YNTNQNMQLK ISQQVLQTLN TLQGSLNNYN SKCSEVIKGV TEELTRNVNT   600
HKAKHDSTLK SLLNITTNLL MNQMNELVRS ISTSLEIFQS DSTSHYRKDL   650
NEIYQSHQQF LKNLQNDIKS CLDSIGSSIL TSINEISQNC TTNLNSMNVL   700
IENQQSGSSK LIKEQDLEIK KLKNDLINER RISNQFNQQL AEMKRYFQDH   750
VSRTRSEFHD ELNKCIDNLK DKQSKLDQDI WQKTASIFNE TDIVVNKIHS   800
DSIASLAHNA ENTLKTVSQN NESFTNDLIS LSRGMNMDIS SKLRSLPINE   850
FLNKISQTIC ETCGDDNTIA SNPVLTSIKK FQNIICSDIA LTNEKIMSLI   900
DEIQSQIETI SNENNINLIA INENFNSLCN FILTDYDENI MQISKTQDEV   950
LSEHCEKLQS LKILGMDIFT AHSIEKPLHE HTRPEASVIK ALPLLDYPKQ   1000
FQIYRDAENK SKDDTSNSRT CIPNLSTNEN FPLSQFSPKT PVPVPDQPLP   1050
KVLIPKSINS AKSNRSKTLP NTEGTGRESQ NNLKRRFTTE PILKGEETEN   1100
NDILQNKKLH Q                                             1111