SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKAP123
Systematic nameYER110C
AliasYRB4
DescriptionImportin subunit beta-4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP40069
SGD YER110C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005643nuclear poreIDA:SGD.
Molecular functionGO:0008320protein carrier activityTAS:SGD.
Biological processGO:0006606protein import into nucleusTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1113
Molecular weight122601

	---------+----------+----------+----------+----------+
MDQQFLSQLE QTLHAITSGV GLKEATKTLQ TQFYTQPTTL PALIHILQNG   50
SDDSLKQLAG VEARKLVSKH WNAIDESTRA SIKTSLLQTA FSEPKENVRH   100
SNARVIASIG TEELDGNKWP DLVPNLIQTA SGEDVQTRQT AIFILFSLLE   150
DFTSSLSGHI DDFLALFSQT INDPSSLEIR SLSAQALNHV SALIEEQETI   200
NPVQAQKFAA SIPSVVNVLD AVIKADDTMN AKLIFNCLND FLLLDSQLTG   250
NFIVDLIKLS LQIAVNSEID EDVRVFALQF IISSLSYRKS KVSQSKLGPE   300
ITVAALKVAC EEIDVDDELN NEDETGENEE NTPSSSAIRL LAFASSELPP   350
SQVASVIVEH IPAMLQSANV FERRAILLAI SVAVTGSPDY ILSQFDKIIP   400
ATINGLKDTE PIVKLAALKC IHQLTTDLQD EVAKFHEEYL PLIIDIIDSA   450
KNIVIYNYAT VALDGLLEFI AYDAIAKYLD PLMNKLFYML ESNESSKLRC   500
AVVSAIGSAA FAAGSAFIPY FKTSVHYLEK FIQNCSQIEG MSEDDIELRA   550
NTFENISTMA RAVRSDAFAE FAEPLVNSAY EAIKTDSARL RESGYAFIAN   600
LAKVYGENFA PFLKTILPEI FKTLELDEYQ FNFDGDAEDL AAFADSANEE   650
ELQNKFTVNT GISYEKEVAS AALSELALGT KEHFLPYVEQ SLKVLNEQVD   700
ESYGLRETAL NTIWNVVKSV LLASKVEPES YPKGIPASSY VNADVLAVIQ   750
AARETSMGNL SDEFETSMVI TVMEDFANMI KQFGAIIIMD NGDSSMLEAL   800
CMQVLSVLKG THTCQTIDIE EDVPRDEELD ASETEATLQD VALEVLVSLS   850
QALAGDFAKV FDNFRPVVFG LFQSKSKNKR SSAVGAASEL ALGMKEQNPF   900
VHEMLEALVI RLTSDKSLEV RGNAAYGVGL LCEYASMDIS AVYEPVLKAL   950
YELLSAADQK ALAAEDDEAT REIIDRAYAN ASGCVARMAL KNSALVPLEQ   1000
TVPALLAHLP LNTGFEEYNP IFELIMKLYQ ENSPVITNET PRIIEIFSAV   1050
FTKENDRIKL EKESTLGREE NMERLKQFQT EEMKHKVIEL LKYLNTTYNG   1100
IVAQNPVLAA VIA                                           1113