SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKAP104
Systematic nameYBR017C
DescriptionImportin subunit beta-2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38217
SGD YBR017C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0008139nuclear localization sequence bindingIPI:SGD.
Biological processGO:0007049cell cycleIMP:SGD.
Biological processGO:0006609mRNA-binding (hnRNP) protein import into nu...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length918
Molecular weight103681

	---------+----------+----------+----------+----------+
MASTWKPAED YVLQLATLLQ NCMSPNPEIR NNAMEAMENF QLQPEFLNYL   50
CYILIEGESD DVLKQHYSLQ DLQNNRATAG MLLKNSMLGG NNLIKSNSHD   100
LGYVKSNIIH GLYNSNNNLV SNVTGIVITT LFSTYYRQHR DDPTGLQMLY   150
QLLELTSNGN EPSIKALSKI MEDSAQFFQL EWSGNTKPME ALLDSFFRFI   200
SNPNFSPVIR SESVKCINTV IPLQTQSFIV RLDKFLEIIF QLAQNDENDL   250
VRAQICISFS FLLEFRPDKL VSHLDGIVQF MLHLITTVNE EKVAIEACEF   300
LHAFATSPNI PEHILQPYVK DIVPILLSKM VYNEESIVLL EASNDDDAFL   350
EDKDEDIKPI APRIVKKKEA GNGEDADDNE DDDDDDDDED GDVDTQWNLR   400
KCSAATLDVM TNILPHQVMD IAFPFLREHL GSDRWFIREA TILALGAMAE   450
GGMKYFNDGL PALIPFLVEQ LNDKWAPVRK MTCWTLSRFS PWILQDHTEF   500
LIPVLEPIIN TLMDKKKDVQ EAAISSVAVF IENADSELVE TLFYSQLLTS   550
FDKCLKYYKK KNLIILYDAI GRFAEKCALD ETAMQIILPP LIEKWALLSD   600
SDKELWPLLE CLSCVASSLG ERFMPMAPEV YNRAFRILCH CVELEAKSHQ   650
DPTIVVPEKD FIITSLDLID GLVQGLGAHS QDLLFPQGTK DLTILKIMLE   700
CLQDPVHEVR QSCFALLGDI VYFFNSELVI GNLEDFLKLI GTEIMHNDDS   750
DGTPAVINAI WALGLISERI DLNTYIIDMS RIILDLFTTN TQIVDSSVME   800
NLSVTIGKMG LTHPEVFSSG AFANDSNWNK WCLSVNALDD VEEKSSAYMG   850
FLKIINLTST EVTMSNDTIH KIVTGLSSNV EANVFAQEIY TFLMNHSAQI   900
SAINFTPDEI SFLQQFTS                                      918