SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameJEN1
Systematic nameYKL217W
DescriptionCarboxylic acid transporter protein homolog

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K9;K338        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36035
SGD YKL217W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005886plasma membraneIMP:SGD.
Molecular functionGO:0015129lactate transporter activityIDA:SGD.
Biological processGO:0015727lactate transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
81Phosphoserine.

Sequence Information
Sequence length616
Molecular weight69376

	---------+----------+----------+----------+----------+
MSSSITDEKI SGEQQQPAGR KLYYNTSTFA EPPLVDGEGN PINYEPEVYN   50
PDHEKLYHNP SLPAQSIQDT RDDELLERVY SQDQGVEYEE DEEDKPNLSA   100
ASIKSYALTR FTSLLHIHEF SWENVNPIPE LRKMTWQNWN YFFMGYFAWL   150
SAAWAFFCVS VSVAPLAELY DRPTKDITWG LGLVLFVRSA GAVIFGLWTD   200
KSSRKWPYIT CLFLFVIAQL CTPWCDTYEK FLGVRWITGI AMGGIYGCAS   250
ATAIEDAPVK ARSFLSGLFF SAYAMGFIFA IIFYRAFGYF RDDGWKILFW   300
FSIFLPILLI FWRLLWPETK YFTKVLKARK LILSDAVKAN GGEPLPKANF   350
KQKMVSMKRT VQKYWLLFAY LVVLLVGPNY LTHASQDLLP TMLRAQLGLS   400
KDAVTVIVVV TNIGAICGGM IFGQFMEVTG RRLGLLIACT MGGCFTYPAF   450
MLRSEKAILG AGFMLYFCVF GVWGILPIHL AELAPADARA LVAGLSYQLG   500
NLASAAASTI ETQLADRYPL ERDASGAVIK EDYAKVMAIL TGSVFIFTFA   550
CVFVGHEKFH RDLSSPVMKK YINQVEEYEA DGLSISDIVE QKTECASVKM   600
IDSNVSKTYE EHIETV                                        616

	* Ubiquitinated lysine is denoted as red color.