SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameITR1
Systematic nameYDR497C
DescriptionMyo-inositol transporter 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K573        
2        Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
purification (6xHis-Myc-ubiquitin), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]

"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP30605
SGD YDR497C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0005365myo-inositol transporter activityIMP:SGD.
Biological processGO:0015798myo-inositol transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
46Phosphoserine.

Sequence Information
Sequence length584
Molecular weight63570

	---------+----------+----------+----------+----------+
MGIHIPYLTS KTSQSNVGDA VGNADSVEFN SEHDSPSKRG KITLESHEIQ   50
RAPASDDEDR IQIKPVNDED DTSVMITFNQ SLSPFIITLT FVASISGFMF   100
GYDTGYISSA LISIGTDLDH KVLTYGEKEI VTAATSLGAL ITSIFAGTAA   150
DIFGRKRCLM GSNLMFVIGA ILQVSAHTFW QMAVGRLIMG FGVGIGSLIA   200
PLFISEIAPK MIRGRLTVIN SLWLTGGQLV AYGCGAGLNY VNNGWRILVG   250
LSLIPTAVQF TCLCFLPDTP RYYVMKGDLA RATEVLKRSY TDTSEEIIER   300
KVEELVTLNQ SIPGKNVPEK VWNTIKELHT VPSNLRALII GCGLQAIQQF   350
TGWNSLMYFS GTIFETVGFK NSSAVSIIVS GTNFIFTLVA FFSIDKIGRR   400
TILLIGLPGM TMALVVCSIA FHFLGIKFDG AVAVVVSSGF SSWGIVIIVF   450
IIVFAAFYAL GIGTVPWQQS ELFPQNVRGI GTSYATATNW AGSLVIASTF   500
LTMLQNITPA GTFAFFAGLS CLSTIFCYFC YPELSGLELE EVQTILKDGF   550
NIKASKALAK KRKQQVARVH ELKYEPTQEI IEDI                    584

	* Ubiquitinated lysine is denoted as red color.