SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameIST2
Systematic nameYBR086C
DescriptionIncreased sodium tolerance protein 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38250
SGD YBR086C
Gene Ontology (GO)
Cellular componentGO:0005933cellular budIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006970response to osmotic stressTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
638Phosphoserine.
701Phosphothreonine.
704Phosphoserine.
720Phosphoserine.
726Phosphothreonine.
729Phosphoserine.
757Phosphoserine.
850Phosphothreonine.
853Phosphothreonine.
856Phosphothreonine.
924Phosphoserine.
926Phosphoserine.

Sequence Information
Sequence length946
Molecular weight105904

	---------+----------+----------+----------+----------+
MSQTITSLDP NCVIVFNKTS SANEKSLNVE FKRLNIHSII EPGHDLQTSY   50
AFIRIHQDNA KPLFSFLQNL DFIESIIPYH DTELSDDLHK LISISKSKIL   100
EAPKQYELYN LSNLTNNPKQ SLYFAFLQNY IKWLIPFSFF GLSIRFLSNF   150
TYEFNSTYSL FAILWTLSFT AFWLYKYEPF WSDRLSKYSS FSTIEFLQDK   200
QKAQKKASSV IMLKKCCFIP VALLFGAILL SFQLYCFALE IFYKQIYNGP   250
MISILSFLPT ILICTFTPVL TVIYNKYFVE PMTKWENHSS VVNAKKSKEA   300
KNFVIIFLSS YVPLLITLFL YLPMGHLLTA EIRTKVFNAF SILARLPTHD   350
SDFIIDTKRY EDQFFYFIVI NQLIQFSMEN FVPSLVSIAQ QKINGPNPNF   400
VKAESEIGKA QLSSSDMKIW SKVKSYQTDP WGATFDLDAN FKKLLLQFGY   450
LVMFSTIWPL APFICLIVNL IVYQVDLRKA VLYSKPEYFP FPIYDKPSSV   500
SNTQKLTVGL WNSVLVMFSI LGCVITATLT YMYQSCNIPG VGAHTSIHTN   550
KAWYLANPIN HSWINIVLYA VFIEHVSVAI FFLFSSILKS SHDDVANGIV   600
PKHVVNVQNP PKQEVFEKIP SPEFNSNNEK ELVQRKGSAN EKLHQELGEK   650
QPASSANGYE AHAATHANND PSSLSSASSP SLSSSSSSSK TGVVKAVDND   700
TAGSAGKKPL ATESTEKRNS LVKVPTVGSY GVAGATLPET IPTSKNYYLR   750
FDEDGKSIRD AKSSAESSNA TNNNTLGTES KLLPDGDAVD ALSRKIDQIP   800
KIAVTGGENN ENTQAKDDAA TKTPLIKDAN IKPVVNAAVN DNQSKVSVAT   850
EQTKKTEVST KNGPSRSIST KETKDSARPS NNNTTTTTTT DATQPHHHHH   900
HHRHRDAGVK NVTNNSKTTE SSSSSSAAKE KPKHKKGLLH KLKKKL       946