SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameINO1
Systematic nameYJL153C
DescriptionInositol-3-phosphate synthase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP11986
SGD YJL153C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:UniProtKB.
Molecular functionGO:0004512inositol-3-phosphate synthase activityIDA:UniProtKB.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006021inositol biosynthetic processIDA:UniProtKB.
EC number 5.5.1.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length533
Molecular weight59642

	---------+----------+----------+----------+----------+
MTEDNIAPIT SVKVVTDKCT YKDNELLTKY SYENAVVTKT ASGRFDVTPT   50
VQDYVFKLDL KKPEKLGIML IGLGGNNGST LVASVLANKH NVEFQTKEGV   100
KQPNYFGSMT QCSTLKLGID AEGNDVYAPF NSLLPMVSPN DFVVSGWDIN   150
NADLYEAMQR SQVLEYDLQQ RLKAKMSLVK PLPSIYYPDF IAANQDERAN   200
NCINLDEKGN VTTRGKWTHL QRIRRDIQNF KEENALDKVI VLWTANTERY   250
VEVSPGVNDT MENLLQSIKN DHEEIAPSTI FAAASILEGV PYINGSPQNT   300
FVPGLVQLAE HEGTFIAGDD LKSGQTKLKS VLAQFLVDAG IKPVSIASYN   350
HLGNNDGYNL SAPKQFRSKE ISKSSVIDDI IASNDILYND KLGKKVDHCI   400
VIKYMKPVGD SKVAMDEYYS ELMLGGHNRI SIHNVCEDSL LATPLIIDLL   450
VMTEFCTRVS YKKVDPVKED AGKFENFYPV LTFLSYWLKA PLTRPGFHPV   500
NGLNKQRTAL ENFLRLLIGL PSQNELRFEE RLL                     533