SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameIML2
Systematic nameYJL082W
DescriptionMitochondrial outer membrane protein IML2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP47031
SGD YJL082W
Gene Ontology (GO)
Cellular componentGO:0005741mitochondrial outer membraneIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
265Phosphoserine.
268Phosphoserine.
380Phosphothreonine.
383Phosphoserine.

Sequence Information
Sequence length731
Molecular weight82536

	---------+----------+----------+----------+----------+
MFRVFGSFGS KGNQSSGEEQ STKTKQVLKQ ANDFEIALKA MDFVLDDRTD   50
EGLNLLKKAE METGSDQTIL TLARGVIEFL QATLSFETEE MKRAAITLGK   100
AEQMSWKSKQ NAEKTNFRSS SIYPPGTVYA VTYTESCLLH ALLMLFSESM   150
MEAAKALLKL RRAYTMLQDI MVTVKKAERS KNSSSPSPSE KSQESCGSFV   200
SAETTFISVD IPYKLSSEDK SNPLLLEFAE KIYTMRMGRL SGAHIGNTPS   250
FHRLRDDLGL QTTPSQASDR HSVSDDFDLE QATIDEFIHS GANLCYGILQ   300
VVLSLLPPAI GAVLSIVGFK GSREEGLRLV WKATKERNVH GCIGLLGLMF   350
YYDGPFQFTD ADFDIPPNDN GSRALNKSRT NDSSLLPGYM DSATLLHPGK   400
ILEDALLKAR ALFPNSALWL LNEAKMLAGK GRLRDSLALM DSIDVNSIRM   450
RQVKSLMVFE RAILLVNLHE YNRAADDLIS LLDISDWSHA LYTYFAGCCY   500
LENWRMTQLG LLNDGKEQFY KERARELIFD APSLLGKKTF KSKNLPLDRF   550
MLRKVQQFNN MQKKLNLQEP LDSIATSPVH ELAYFYNGYN RMTENDLILT   600
KKMLTEYHNP AIDSEDPDQE LIRNLLLSLT LRRLGDAERG LALLDDIVLP   650
KIFYIQNGKV KYFKKTEDPW AYPAALYERA LFCWKLGGME SLNECREWLL   700
RAQNYAADYE LSTRIGMKIK AALDRVENAL A                       731