SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameILV5
Systematic nameYLR355C
DescriptionKetol-acid reductoisomerase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP06168
SGD YLR355C
Gene Ontology (GO)
Cellular componentGO:0042645mitochondrial nucleoidIDA:SGD.
Molecular functionGO:0004455ketol-acid reductoisomerase activityTAS:SGD.
Biological processGO:0009082branched chain family amino acid biosynthet...TAS:SGD.
Biological processGO:0000002mitochondrial genome maintenanceIMP:SGD.
EC number 1.1.1.86

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length395
Molecular weight44368

	---------+----------+----------+----------+----------+
MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ   50
INFGGTVETV YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG   100
LNVIIGVRKD GASWKAAIED GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ   150
SETWPAIKPL LTKGKTLYFS HGFSPVFKDL THVEPPKDLD VILVAPKGSG   200
RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS GYVYQTTFER   250
EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL   300
YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT   350
ETKRSLEFNS QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ        395