SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameILS1
Systematic nameYBL076C
DescriptionIsoleucyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP09436
SGD YBL076C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0004822isoleucine-tRNA ligase activityIDA:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number 6.1.1.5

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1059Phosphoserine.

Sequence Information
Sequence length1072
Molecular weight122984

	---------+----------+----------+----------+----------+
MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT   50
GTPHYGHILA STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG   100
ITGKDDVFKY GLENYNNECR SIVMTYASDW RKTIGRLGRW IDFDNDYKTM   150
YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY STGLTTPLSN FEAQQNYKDV   200
NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD FEYVKIYDET   250
RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ   300
FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP   350
NAIDDLGRFT KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF   400
CWRSDTPLLY RSVPAWFVRV KNIVPQMLDS VMKSHWVPNT IKEKRFANWI   450
ANARDWNVSR NRYWGTPIPL WVSDDFEEVV CVGSIKELEE LTGVRNITDL   500
HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ HYPFENTEKF   550
DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG   600
RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV   650
VSKVLLPWWN SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM   700
QSLVQFIHEE MGQYKLYTVV PKLLNFIDEL TNWYIRFNRR RLKGENGVED   750
CLKALNSLFD ALFTFVRAMA PFTPFLSESI YLRLKEYIPE AVLAKYGKDG   800
RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK KTISLKTPLK   850
TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW   900
PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI   950
RGLPESAVQA GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK   1000
KCGLEATDDV LVEYELVKDT IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT   1050
DPIGDEEQSI NDTIFKLKVF KL                                 1072