SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameIDI1
Systematic nameYPL117C
AliasBOT2
DescriptionIsopentenyl-diphosphate Delta-isomerase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP15496
SGD YPL117C
Gene Ontology (GO)
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
EC number 5.3.3.2

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
24Phosphothreonine.

Sequence Information
Sequence length288
Molecular weight33352

	---------+----------+----------+----------+----------+
MTADNNSMPH GAVSSYAKLV QNQTPEDILE EFPEIIPLQQ RPNTRSSETS   50
NDESGETCFS GHDEEQIKLM NENCIVLDWD DNAIGAGTKK VCHLMENIEK   100
GLLHRAFSVF IFNEQGELLL QQRATEKITF PDLWTNTCCS HPLCIDDELG   150
LKGKLDDKIK GAITAAVRKL DHELGIPEDE TKTRGKFHFL NRIHYMAPSN   200
EPWGEHEIDY ILFYKINAKE NLTVNPNVNE VRDFKWVSPN DLKTMFADPS   250
YKFTPWFKII CENYLFNWWE QLDDLSEVEN DRQIHRML                288