SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHXT7
Systematic nameYDR342C
DescriptionHigh-affinity hexose transporter HXT6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K40;K318;K560        
2K40;K268;K560        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39004
SGD YDR342C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0005353fructose transporter activityTAS:SGD.
Molecular functionGO:0005355glucose transporter activityIDA:SGD.
Molecular functionGO:0015578mannose transporter activityTAS:SGD.
Molecular functionGO:0015146pentose transporter activityIMP:SGD.
Biological processGO:0008645hexose transportTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length570
Molecular weight62735

	---------+----------+----------+----------+----------+
MSQDAAIAEQ TPVEHLSAVD SASHSVLSTP SNKAERDEIK AYGEGEEHEP   50
VVEIPKRPAS AYVTVSIMCI MIAFGGFVFG WDTGTISGFI NQTDFIRRFG   100
MKHKDGTNYL SKVRTGLIVS IFNIGCAIGG IILSKLGDMY GRKVGLIVVV   150
VIYIIGIIIQ IASINKWYQY FIGRIISGLG VGGIAVLSPM LISEVSPKHL   200
RGTLVSCYQL MITAGIFLGY CTNFGTKNYS NSVQWRVPLG LCFAWALFMI   250
GGMTFVPESP RYLAEVGKIE EAKRSIAVSN KVAVDDPSVL AEVEAVLAGV   300
EAEKLAGNAS WGELFSSKTK VLQRLIMGAM IQSLQQLTGD NYFFYYGTTI   350
FKAVGLSDSF ETSIVLGIVN FASTFVGIYV VERYGRRTCL LWGAASMTAC   400
MVVYASVGVT RLWPNGQDQP SSKGAGNCMI VFACFYIFCF ATTWAPIPYV   450
VVSETFPLRV KSKAMSIATA ANWLWGFLIG FFTPFITGAI NFYYGYVFMG   500
CLVFMFFYVL LVVPETKGLT LEEVNTMWEE GVLPWKSASW VPPSRRGANY   550
DAEEMTHDDK PLYKRMFSTK                                     570

	* Ubiquitinated lysine is denoted as red color.