SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHXT3
Systematic nameYDR345C
DescriptionLow-affinity glucose transporter HXT3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32466
SGD YDR345C
Gene Ontology (GO)
Molecular functionGO:0005353fructose transporter activityTAS:SGD.
Molecular functionGO:0005355glucose transporter activityIDA:SGD.
Molecular functionGO:0015578mannose transporter activityTAS:SGD.
Biological processGO:0008645hexose transportTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
23Phosphoserine.

Sequence Information
Sequence length567
Molecular weight62558

	---------+----------+----------+----------+----------+
MNSTPDLISP QKSSENSNAD LPSNSSQVMN MPEEKGVQDD FQAEADQVLT   50
NPNTGKGAYV TVSICCVMVA FGGFVFGWDT GTISGFVAQT DFLRRFGMKH   100
KDGSYYLSKV RTGLIVSIFN IGCAIGGIIL AKLGDMYGRK MGLIVVVVIY   150
IIGIIIQIAS INKWYQYFIG RIISGLGVGG IAVLSPMLIS EVAPKEMRGT   200
LVSCYQLMIT LGIFLGYCTN FGTKNYSNSV QWRVPLGLCF AWALFMIGGM   250
TFVPESPRYL VEAGQIDEAR ASLSKVNKVA PDHPFIQQEL EVIEASVEEA   300
RAAGSASWGE LFTGKPAMFK RTMMGIMIQS LQQLTGDNYF FYYGTTVFNA   350
VGMSDSFETS IVFGVVNFFS TCCSLYTVDR FGRRNCLLYG AIGMVCCYVV   400
YASVGVTRLW PNGEGNGSSK GAGNCMIVFA CFYIFCFATT WAPIAYVVIS   450
ETFPLRVKSK AMSIATAANW LWGFLIGFFT PFITGAINFY YGYVFMGCMV   500
FAYFYVFFFV PETKGLTLEE VNDMYAEGVL PWKSASWVPT SQRGANYDAD   550
ALMHDDQPFY KKMFGKK                                       567