SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHXK2
Systematic nameYGL253W
AliasHEX1, HKB
DescriptionHexokinase-2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP04807
SGD YGL253W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004396hexokinase activityIDA:SGD.
Biological processGO:0032445fructose importIGI:SGD.
Biological processGO:0006000fructose metabolic processIMP:SGD.
Biological processGO:0046323glucose importIGI:SGD.
Biological processGO:0006096glycolysisIDA:SGD.
Biological processGO:0006013mannose metabolic processIDA:SGD.
Biological processGO:0008361regulation of cell sizeIMP:SGD.
Biological processGO:0046015regulation of transcription by glucoseIDA:SGD.
Biological processGO:0001302replicative cell agingIMP:SGD.
EC number 2.7.1.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
15Phosphoserine.
158Phosphoserine.
396Phosphoserine.

Sequence Information
Sequence length486
Molecular weight53942

	---------+----------+----------+----------+----------+
MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS   50
ELEKGLSKKG GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG   100
GDRTFDTTQS KYRLPDAMRT TQNPDELWEF IADSLKAFID EQFPQGISEP   150
IPLGFTFSFP ASQNKINEGI LQRWTKGFDI PNIENHDVVP MLQKQITKRN   200
IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY YDVCSDIEKL   250
QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT   300
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI   350
EEDPFENLED TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI   400
AAICQKRGYK TGHIAADGSV YNRYPGFKEK AANALKDIYG WTQTSLDDYP   450
IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS VGIIGA                  486