SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHXK1
Systematic nameYFR053C
AliasHKA
DescriptionHexokinase-1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP04806
SGD YFR053C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0004396hexokinase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0032445fructose importIGI:SGD.
Biological processGO:0006000fructose metabolic processIMP:SGD.
Biological processGO:0046323glucose importIGI:SGD.
Biological processGO:0006096glycolysisIDA:SGD.
Biological processGO:0006013mannose metabolic processIDA:SGD.
EC number 2.7.1.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
15Phosphoserine.

Sequence Information
Sequence length485
Molecular weight53738

	---------+----------+----------+----------+----------+
MVHLGPKKPQ ARKGSMADVP KELMDEIHQL EDMFTVDSET LRKVVKHFID   50
ELNKGLTKKG GNIPMIPGWV MEFPTGKESG NYLAIDLGGT NLRVVLVKLS   100
GNHTFDTTQS KYKLPHDMRT TKHQEELWSF IADSLKDFMV EQELLNTKDT   150
LPLGFTFSYP ASQNKINEGI LQRWTKGFDI PNVEGHDVVP LLQNEISKRE   200
LPIEIVALIN DTVGTLIASY YTDPETKMGV IFGTGVNGAF YDVVSDIEKL   250
EGKLADDIPS NSPMAINCEY GSFDNEHLVL PRTKYDVAVD EQSPRPGQQA   300
FEKMTSGYYL GELLRLVLLE LNEKGLMLKD QDLSKLKQPY IMDTSYPARI   350
EDDPFENLED TDDIFQKDFG VKTTLPERKL IRRLCELIGT RAARLAVCGI   400
AAICQKRGYK TGHIAADGSV YNKYPGFKEA AAKGLRDIYG WTGDASKDPI   450
TIVPAEDGSG AGAAVIAALS EKRIAEGKSL GIIGA                   485