SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHUL5
Systematic nameYGL141W
DescriptionProbable E3 ubiquitin-protein ligase HUL5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP53119
SGD YGL141W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
EC number 6.3.2.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length910
Molecular weight105566

	---------+----------+----------+----------+----------+
MLNFTGQTRR RNVNLGNRTR NSKKDLLEKA KRERERRAQD KLKEDASKTI   50
QKSIRRHFSN VRLFKNTFTS SQLVHMIPAY GGKLIYYISQ YDLQQLLKLS   100
HNFLSSYPNS LGNRQLLSLL KLYQDDALVA ETLSDLNMDC PTVDEFLDSL   150
SVYLCRASFL SYSSASKLAD VIEAWEVMHS SASISIFSIS IGSYEKRPFA   200
LQFYCILAER NLLPQLINTN PILWDNMAKT YSHCSKGGQK NIAKLLIPNF   250
NNHIAPSVLR SDNDYVLKFY EKAFIDEVIA TTANYVSDED HVKNLMCYIA   300
SSPNQSCKNS VLITLLSNKD FVRRLSWEFF HTKFNASKTE AHPLFSVLAQ   350
LIDMHLLIST DRELLDYNSV IPIEELKKFT STLKDFTFRQ YWELPKSERN   400
PMLKEAVPLL SKVYERDSRL HFLSTENNPT YWENSEKQFL NLRFYEELQE   450
YEDLYREHLE EESDEDMEKE IDLDKERPPL KSLLLNKMKK RLKSSLRFRK   500
LEILLELPFF IPFEERVDLF YMFIALDKKR LSLDDDHNLI NMFTPWASTG   550
MRKQSAIISR DNVLEDAFNA FNSIGERFKA SLDVTFINEF GEEAGIDGGG   600
ITKEFLTTVS DEGFKDPKHE LFRTNDRYEL YPSVVYDATK LKYIWFLGKV   650
VGKCLYEHVL IDVSFADFFL KKLLNYSNGF LSSFSDLGSY DSVLYNNLIK   700
LLNMTTDEIK SLDLTFEIDE PESSAKVVDL IPNGSKTYVT KDNVLLYVTK   750
VTDYKLNKRC FKPVSAFHGG LSVIIAPHWM EMFNSIELQM LISGERDNID   800
LDDLKSNTEY GGYKEEDQTI VDFWEVLNEF KFEEKLNFLK FVTSVPQAPL   850
QGFKALDPKF GIRNAGTEKY RLPTASTCVN LLKLPDYRNK TILREKLLYA   900
INSGARFDLS                                                910