SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHTB2
Systematic nameYBL002W
AliasH2B2
DescriptionHistone H2B.2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K124MONORAD6      
2K124 RAD6      
3K124MONORAD6RINGSPRFBRE1   
4K124        
5K124MONORAD6    UBP8 
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Rad6-dependent ubiquitination of histone H2B in yeast."
Robzyk K, Recht J, Osley MA.
Science. 287(5452):501-4 (2000) [10642555]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Ubiquitination of histone H2B by Rad6 is required for efficient Dot1-mediated methylation of histone H3 lysine 79."
Ng HH, Xu RM, Zhang Y, Struhl K.
J Biol Chem. 277(38):34655-7 (2002) [12167634]
[3]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"A conserved RING finger protein required for histone H2B monoubiquitination and cell size control."
Hwang WW, Venkatasubrahmanyam S, Ianculescu AG, Tong A, Boone C, Madhani HD.
Mol Cell. 11(1):261-6 (2003) [12535538]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[5]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Deubiquitination of histone H2B by a yeast acetyltransferase complex regulates transcription."
Daniel JA, Torok MS, Sun ZW, Schieltz D, Allis CD, Yates JR 3rd, Grant PA.
J Biol Chem. 279(3):1867-71 (2004) [14660634]

Cross References
Swiss-Prot accessionP02294
SGD YBL002W
Gene Ontology (GO)
Cellular componentGO:0000788nuclear nucleosomeTAS:SGD.
Molecular functionGO:0003677DNA bindingTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:UniProtKB.
Biological processGO:0006333chromatin assembly or disassemblyTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
7N6-acetyllysine, alternate.
8N6-acetyllysine, alternate.
11Phosphoserine.
12N6-acetyllysine.
17N6-acetyllysine, alternate.
40Phosphothreonine.

Sequence Information
Sequence length131
Molecular weight14237

	---------+----------+----------+----------+----------+
MSSAAEKKPA SKAPAEKKPA AKKTSTSVDG KKRSKVRKET YSSYIYKVLK   50
QTHPDTGISQ KSMSILNSFV NDIFERIATE ASKLAAYNKK STISAREIQT   100
AVRLILPGEL AKHAVSEGTR AVTKYSSSTQ A                       131

	* Ubiquitinated lysine is denoted as red color.