SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHTB1
Systematic nameYDR224C
AliasH2B1, SPT12
DescriptionHistone H2B.1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K124        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP02293
SGD YDR224C
Gene Ontology (GO)
Cellular componentGO:0000788nuclear nucleosomeIDA:SGD.
Molecular functionGO:0003677DNA bindingIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:UniProtKB.
Biological processGO:0006333chromatin assembly or disassemblyIDA:SGD.
Biological processGO:0045816negative regulation of global transcription...IMP:SGD.
Biological processGO:0006301postreplication repairIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
7N6-acetyllysine, alternate.
8N6-acetyllysine, alternate.
11Phosphoserine.
12N6-acetyllysine.
17N6-acetyllysine, alternate.

Sequence Information
Sequence length131
Molecular weight14252

	---------+----------+----------+----------+----------+
MSAKAEKKPA SKAPAEKKPA AKKTSTSTDG KKRSKARKET YSSYIYKVLK   50
QTHPDTGISQ KSMSILNSFV NDIFERIATE ASKLAAYNKK STISAREIQT   100
AVRLILPGEL AKHAVSEGTR AVTKYSSSTQ A                       131

	* Ubiquitinated lysine is denoted as red color.