SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHST3
Systematic nameYOR025W
DescriptionNAD-dependent histone deacetylase HST3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP53687
SGD YOR025W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIC:SGD.
Biological processGO:0006348chromatin silencing at telomereIGI:SGD.
Biological processGO:0016575histone deacetylationIMP:SGD.
Biological processGO:0046459short-chain fatty acid metabolic processIMP:SGD.
EC number 3.5.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length447
Molecular weight50524

	---------+----------+----------+----------+----------+
MTSVSPSPPA SRSGSMCSDL PSSLQTEKLA HIIGLDADDE VLRRVTKQLS   50
RSRRIACLTG AGISCNAGIP DFRSSDGLYD LVKKDCSQYW SIKSGREMFD   100
ISLFRDDFKI SIFAKFMERL YSNVQLAKPT KTHKFIAHLK DRNKLLRCYT   150
QNIDGLEESI GLTLSNRKLP LTSFSSHWKN LDVVQLHGDL KTLSCTKCFQ   200
TFPWSRYWSR CLRRGELPLC PDCEALINKR LNEGKRTLGS NVGILRPNIV   250
LYGENHPSCE IITQGLNLDI IKGNPDFLII MGTSLKVDGV KQLVKKLSKK   300
IHDRGGLIIL VNKTPIGESS WHGIIDYQIH SDCDNWVTFL ESQIPDFFKT   350
QDQIKKLRQL KREASDLRKQ MKAQKDSIGT PPTTPLRTAQ GIDIQGNNEL   400
NTKIKSLNTV KRKILSPENS SEEDEEENLD TRKRAKIRPT FGDNQAS      447