SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHSP42
Systematic nameYDR171W
DescriptionHeat shock protein 42

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ12329
SGD YDR171W
Gene Ontology (GO)
Cellular componentGO:0005832chaperonin-containing T-complexIPI:SGD.
Cellular componentGO:0005856cytoskeletonIPI:SGD.
Molecular functionGO:0051082unfolded protein bindingTAS:SGD.
Biological processGO:0007010cytoskeleton organization and biogenesisIGI:SGD.
Biological processGO:0006950response to stressIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
213Phosphoserine.
214Phosphoserine.
215Phosphoserine.

Sequence Information
Sequence length375
Molecular weight42817

	---------+----------+----------+----------+----------+
MSFYQPSLSL YDVLNALSNQ TGQRGQQGYP RQPQRPQRYH PHYGQVHVGG   50
HHPRHHPLYS RYNGVPNTYY YQFPGQAYYY SPEYGYDDED GEEEDQDEDM   100
VGDSGTTRQE DGGEDSNSRR YPSYYHCNTA RNNRTNQQAN SLNDLLTALI   150
GVPPYEGTEP EIEANTEQEG EKGEEKDKKD KSEAPKEEAG ETNKEKPLNQ   200
LEESSRPPLA KKSSSFAHLQ APSPIPDPLQ VSKPETRMDL PFSPEVNVYD   250
TEDTYVVVLA LPGANSRAFH IDYHPSSHEM LIKGKIEDRV GIDEKFLKIT   300
ELKYGAFERT VKFPVLPRIK DEEIKATYNN GLLQIKVPKI VNDTEKPKPK   350
KRIAIEEIPD EELEFEENPN PTVEN                              375