SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHSP30
Systematic nameYCR021C
Description30 kDa heat shock protein

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25619
SGD YCR021C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Biological processGO:0006950response to stressIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
308Phosphoserine.
312Phosphoserine.
331Phosphothreonine.

Sequence Information
Sequence length332
Molecular weight37045

	---------+----------+----------+----------+----------+
MNDTLSSFLN RNEALGLNPP HGLDMHITKR GSDWLWAVFA VFGFILLCYV   50
VMFFIAENKG SRLTRYALAP AFLITFFEFF AFFTYASDLG WTGVQAEFNH   100
VKVSKSITGE VPGIRQIFYS KYIAWFLSWP CLLFLIELAA STTGENDDIS   150
ALDMVHSLLI QIVGTLFWVV SLLVGSLIKS TYKWGYYTIG AVAMLVTQGV   200
ICQRQFFNLK TRGFNALMLC TCMVIVWLYF ICWGLSDGGN RIQPDGEAIF   250
YGVLDLCVFA IYPCYLLIAV SRDGKLPRLS LTGGFSHHHA TDDVEDAAPE   300
TKEAVPESPR ASGETAIHEP EPEAEQAVED TA                      332