SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameHSL1
Systematic nameYKL101W
DescriptionProbable serine/threonine-protein kinase HSL1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDC20   
2         
3   RINGAPCCDH1   
4   RINGSCFCDC4   
5        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Hsl1p, a Swe1p inhibitor, is degraded via the anaphase-promoting complex."
Burton JL, Solomon MJ.
Mol Cell Biol. 20(13):4614-25 (2000) [10848588]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Hsl1p, a Swe1p inhibitor, is degraded via the anaphase-promoting complex."
Burton JL, Solomon MJ.
Mol Cell Biol. 20(13):4614-25 (2000) [10848588]
[4]EvidenceProtein Stability Assay
MethodWestern blot
Reference"GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down-regulation by Ca2+ in budding yeast."
Mizunuma M, Hirata D, Miyaoka R, Miyakawa T.
EMBO J. 20(5):1074-85 (2001) [11230131]
[5]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP34244
SGD YKL101W
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005940septin ringIDA:SGD.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0000078cell morphogenesis checkpointTAS:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIGI:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
Biological processGO:0000135septin checkpointIGI:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
57Phosphoserine.
629Phosphoserine.
643Phosphoserine.
1220Phosphoserine.
1284Phosphoserine.
1287Phosphoserine.
1325Phosphoserine.
1328Phosphoserine.
1329Phosphoserine.

Sequence Information
Sequence length1518
Molecular weight169593

	---------+----------+----------+----------+----------+
MTGHVSKTSH VPKGRPSSLA KKAAKRAMAK VNSNPKRASG HLERVVQSVN   50
DATKRLSQPD STVSVATKSS KRKSRDTVGP WKLGKTLGKG SSGRVRLAKN   100
METGQLAAIK IVPKKKAFVH CSNNGTVPNS YSSSMVTSNV SSPSIASREH   150
SNHSQTNPYG IEREIVIMKL ISHTNVMALF EVWENKSELY LVLEYVDGGE   200
LFDYLVSKGK LPEREAIHYF KQIVEGVSYC HSFNICHRDL KPENLLLDKK   250
NRRIKIADFG MAALELPNKL LKTSCGSPHY ASPEIVMGRP YHGGPSDVWS   300
CGIVLFALLT GHLPFNDDNI KKLLLKVQSG KYQMPSNLSS EARDLISKIL   350
VIDPEKRITT QEILKHPLIK KYDDLPVNKV LRKMRKDNMA RGKSNSDLHL   400
LNNVSPSIVT LHSKGEIDES ILRSLQILWH GVSRELITAK LLQKPMSEEK   450
LFYSLLLQYK QRHSISLSSS SENKKSATES SVNEPRIEYA SKTANNTGLR   500
SENNDVKTLH SLEIHSEDTS TVNQNNAITG VNTEINAPVL AQKSQFSINT   550
LSQPESDKAE AEAVTLPPAI PIFNASSSRI FRNSYTSISS RSRRSLRLSN   600
SRLSLSASTS RETVHDNEMP LPQLPKSPSR YSLSRRAIHA SPSTKSIHKS   650
LSRKNIAATV AARRTLQNSA SKRSLYSLQS ISKRSLNLND LLVFDDPLPS   700
KKPASENVNK SEPHSLESDS DFEILCDQIL FGNALDRILE EEEDNEKERD   750
TQRQRQNDTK SSADTFTISG VSTNKENEGP EYPTKIEKNQ FNMSYKPSEN   800
MSGLSSFPIF EKENTLSSSY LEEQKPKRAA LSDITNSFNK MNKQEGMRIE   850
KKIQREQLQK KNDRPSPLKP IQHQELRVNS LPNDQGKPSL SLDPRRNISQ   900
PVNSKVESLL QGLKFKKEPA SHWTHERGSL FMSEHVEDEK PVKASDVSIE   950
SSYVPLTTVA TSSRDPSVLA ESSTIQKPML SLPSSFLNTS MTFKNLSQIL   1000
ADDGDDKHLS VPQNQSRSVA MSHPLRKQSA KISLTPRSNL NANLSVKRNQ   1050
GSPGSYLSND LDGISDMTFA MEIPTNTFTA QAIQLMNNDT DNNKINTSPK   1100
ASSFTKEKVI KSAAYISKEK EPDNSDTNYI PDYTIPNTYD EKAINIFEDA   1150
PSDEGSLNTS SSESDSRASV HRKAVSIDTM ATTNVLTPAT NVRVSLYWNN   1200
NSSGIPRETT EEILSKLRLS PENPSNTHMQ KRFSSTRGSR DSNALGISQS   1250
LQSMFKDLEE DQDGHTSQAD ILESSMSYSK RRPSEESVNP KQRVTMLFDE   1300
EEEESKKVGG GKIKEEHTKL DNKISEESSQ LVLPVVEKKE NANNTENNYS   1350
KIPKPSTIKV TKDTAMESNT QTHTKKPILK SVQNVEVEEA PSSDKKNWFV   1400
KLFQNFSSHN NATKASKNHV TNISFDDAHM LTLNEFNKNS IDYQLKNLDH   1450
KFGRKVVEYD CKFVKGNFKF KIKITSTPNA SSVITVKKRS KHSNTSSNKA   1500
FEKFNDDVER VIRNAGRS                                      1518