SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHRP1
Systematic nameYOL123W
AliasNAB4, NAB5
DescriptionNuclear polyadenylated RNA-binding protein 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ99383
SGD YOL123W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005849mRNA cleavage factor complexIPI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIDA:SGD.
Biological processGO:0006379mRNA cleavageIDA:SGD.
Biological processGO:0006378mRNA polyadenylationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2Phosphoserine.
3Phosphoserine.

Sequence Information
Sequence length534
Molecular weight59650

	---------+----------+----------+----------+----------+
MSSDEEDFND IYGDDKPTTT EEVKKEEEQN KAGSGTSQLD QLAALQALSS   50
SLNKLNNPNS NNSSSNNSNQ DTSSSKQDGT ANDKEGSNED TKNEKKQESA   100
TSANANANAS SAGPSGLPWE QLQQTMSQFQ QPSSQSPPQQ QVTQTKEERS   150
KADLSKESCK MFIGGLNWDT TEDNLREYFG KYGTVTDLKI MKDPATGRSR   200
GFGFLSFEKP SSVDEVVKTQ HILDGKVIDP KRAIPRDEQD KTGKIFVGGI   250
GPDVRPKEFE EFFSQWGTII DAQLMLDKDT GQSRGFGFVT YDSADAVDRV   300
CQNKFIDFKD RKIEIKRAEP RHMQQKSSNN GGNNGGNNMN RRGGNFGNQG   350
DFNQMYQNPM MGGYNPMMNP QAMTDYYQKM QEYYQQMQKQ TGMDYTQMYQ   400
QQMQQMAMMM PGFAMPPNAM TLNQPQQDSN ATQGSPAPSD SDNNKSNDVQ   450
TIGNTSNTDS GSPPLNLPNG PKGPSQYNDD HNSGYGYNRD RGDRDRNDRD   500
RDYNHRSGGN HRRNGRGGRG GYNRRNNGYH PYNR                    534