SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHPR1
Systematic nameYDR138W
DescriptionTHO complex subunit HPR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  UBC4HECTNARSP5  Proteasomal Degradation
2 MULTI HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"The mRNA nuclear export factor Hpr1 is regulated by Rsp5-mediated ubiquitylation."
Gwizdek C, Hobeika M, Kus B, Ossareh-Nazari B, Dargemont C, Rodriguez MS.
J Biol Chem. 280(14):13401-5 (2005) [15713680]
[2]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]

Cross References
Swiss-Prot accessionP17629
SGD YDR138W
Gene Ontology (GO)
Cellular componentGO:0016593Cdc73/Paf1 complexIPI:SGD.
Cellular componentGO:0000347THO complexIMP:SGD.
Molecular functionGO:0003676nucleic acid bindingIDA:SGD.
Biological processGO:0006310DNA recombinationIMP:SGD.
Biological processGO:0006406mRNA export from nucleusIMP:SGD.
Biological processGO:0006974response to DNA damage stimulusIMP:SGD.
Biological processGO:0006368RNA elongation from RNA polymerase II promoterIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
234Phosphoserine.
675Phosphoserine, by ATM or ATR.

Sequence Information
Sequence length752
Molecular weight87829

	---------+----------+----------+----------+----------+
MSNTEELIQN SIGFLQKTFK ALPVSFDSIR HEPLPSSMLH ASVLNFEWEP   50
LEKNISAIHD RDSLIDIILK RFIIDSMTNA IEDEEENNLE KGLLNSCIGL   100
DFVYNSRFNR SNPASWGNTF FELFSTIIDL LNSPSTFLKF WPYAESRIEW   150
FKMNTSVEPV SLGESNLISY KQPLYEKLRH WNDILAKLEN NDILNTVKHY   200
NMKYKLENFL SELLPINEES NFNRSASISA LQESDNEWNR SARERESNRS   250
SDVIFAADYN FVFYHLIICP IEFAFSDLEY KNDVDRSLSP LLDAILEIEE   300
NFYSKIKMNN RTRYSLEEAL NTEYYANYDV MTPKLPVYMK HSNAMKMDRN   350
EFWANLQNIK ESDDYTLRPT IMDISLSNTT CLYKQLTQED DDYYRKQFIL   400
QLCFTTNLIR NLISSDETRN FYKSCYLREN PLSDIDFENL DEVNKKRGLN   450
LCSYICDNRV LKFYKIKDPD FYRVIRKLMS SDEKFTTAKI DGFKEFQNFR   500
ISKEKIPPPA FDETFKKFTF IKMGNKLINN VWKIPTGLDK IEQEVKKPEG   550
VYEAAQAKWE SKISSETSGG EAKDEIIRQW QTLRFLRSRY LFDFDKVNEK   600
TGVDGLFEEP RKVEALDDSF KEKLLYKINQ EHRKKLQDAR EYKIGKERKK   650
RALEEEASFP EREQKIKSQR INSASQTEGD ELKSEQTQPK GEISEENTKI   700
KSSEVSSQDP DSGVAGEFAP QNTTAQLENP KTEDNNAATS NISNGSSTQD   750
MK                                                       752