SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHO
Systematic nameYDL227C
DescriptionHomothallic switching endonuclease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTIRAD6RINGSPRFRAD18  Proteasomal Degradation
2 MULTICDC34RINGSCFUFO1  Proteasomal Degradation
3   RINGSCFUFO1   
4 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system."
Kaplun L, Ivantsiv Y, Kornitzer D, Raveh D.
Proc Natl Acad Sci U S A. 97(18):10077-82 (2000) [10963670]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system."
Kaplun L, Ivantsiv Y, Kornitzer D, Raveh D.
Proc Natl Acad Sci U S A. 97(18):10077-82 (2000) [10963670]
[3]EvidenceInteraction Assay
Methodwith substrate receptor
Reference"DNA damage response-mediated degradation of Ho endonuclease via the ubiquitin system involves its nuclear export."
Kaplun L, Ivantsiv Y, Bakhrat A, Raveh D.
J Biol Chem. 278(49):48727-34 (2003) [14506225]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP09932
SGD YDL227C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusTAS:SGD.
Molecular functionGO:0004519endonuclease activityIDA:SGD.
Biological processGO:0000728gene conversion at mating-type locus, DNA d...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length586
Molecular weight66089

	---------+----------+----------+----------+----------+
MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI   50
QQKTKHRAFE GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS   100
GRNATKYKVR WRNLQQCQTL DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE   150
EMERSKGEYF NFDIEVRDLD YLDAQLRISS CIRFGPVLAG NGVLSKFLTG   200
RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM ESLRENAKIW   250
GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF   300
KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI   350
QTVYSSIMDG IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA   400
GGTTSQNVLS YCRSGHKTRE VPPIIKREPV YFSFTDDFQG ESTVYGLTIE   450
GHKNFLLGNK IEVKSCRGCC VGEQLKISQK KNLKHCVACP RKGIKYFYKD   500
WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK GEKLGITPEG   550
LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC                  586