SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHMLALPHA2
Systematic nameYCL067C
AliasALPHA-2, MAT2A, MATAL2
DescriptionMating-type protein ALPHA2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  UBC4, UBC5, UBC6, UBC7      
2  UBC6, UBC7RINGSPRFSSM4   
3 MULTI(K48-linked)       
* Blank: not determined, NA: not applicable.

References
[1]Evidence 
Method 
Reference"Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor."
Chen P, Johnson P, Sommer T, Jentsch S, Hochstrasser M.
Cell. 74(2):357-69 (1993) [8393731]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation."
Swanson R, Locher M, Hochstrasser M.
Genes Dev. 15(20):2660-74 (2001) [11641273]
[3]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"The short-lived MAT alpha 2 transcriptional regulator is ubiquitinated in vivo."
Hochstrasser M, Ellison MJ, Chau V, Varshavsky A.
Proc Natl Acad Sci U S A. 88(11):4606-10 (1991) [1647011]

Cross References
Swiss-Prot accessionQ6B2C0
SGD YCL067C
Gene Ontology (GO)
Biological processGO:0007535donor selectionTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length210
Molecular weight24282

	---------+----------+----------+----------+----------+
MNKIPIKDLL NPQITDEFKS SILDINKKLF SICCNLPKLP ESVTTEEEVE   50
LRDILGFLSR ANKNRKISDE EKKLLQTTSQ LTTTITVLLK EMRSIENDRS   100
NYQLTQKNKS ADGLVFNVVT QDMINKSTKP YRGHRFTKEN VRILESWFAK   150
NIENPYLDTK GLENLMKNTS LSRIQIKNWV SNRRRKEKTI TIAPELADLL   200
SGEPLAKKKE                                                210