SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHMG2
Systematic nameYLR450W
Description3-hydroxy-3-methylglutaryl-coenzyme A reductase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGSPRFHRD1   
2  UBC7      
* Blank: not determined, NA: not applicable.

References
[1]Evidence 
Method 
Reference"Role of 26S proteasome and HRD genes in the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein."
Hampton RY, Gardner RG, Rine J.
Mol Biol Cell. 7(12):2029-44 (1996) [8970163]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ubiquitin-mediated regulation of 3-hydroxy-3-methylglutaryl-CoA reductase."
Hampton RY, Bhakta H.
Proc Natl Acad Sci U S A. 94(24):12944-8 (1997) [9371780]

Cross References
Swiss-Prot accessionP12684
SGD YLR450W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneTAS:SGD.
Cellular componentGO:0005635nuclear envelopeTAS:SGD.
EC number 1.1.1.34

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
565Phosphothreonine.

Sequence Information
Sequence length1045
Molecular weight115692

	---------+----------+----------+----------+----------+
MSLPLKTIVH LVKPFACTAR FSARYPIHVI VVAVLLSAAA YLSVTQSYLN   50
EWKLDSNQYS TYLSIKPDEL FEKCTHYYRS PVSDTWKLLS SKEAADIYTP   100
FHYYLSTISF QSKDNSTTLP SLDDVIYSVD HTRYLLSEEP KIPTELVSEN   150
GTKWRLRNNS NFILDLHNIY RNMVKQFSNK TSEFDQFDLF IILAAYLTLF   200
YTLCCLFNDM RKIGSKFWLS FSALSNSACA LYLSLYTTHS LLKKPASLLS   250
LVIGLPFIVV IIGFKHKVRL AAFSLQKFHR ISIDKKITVS NIIYEAMFQE   300
GAYLIRDYLF YISSFIGCAI YARHLPGLVN FCILSTFMLV FDLLLSATFY   350
SAILSMKLEI NIIHRSTVIR QTLEEDGVVP TTADIIYKDE TASEPHFLRS   400
NVAIILGKAS VIGLLLLINL YVFTDKLNAT ILNTVYFDST IYSLPNFINY   450
KDIGNLSNQV IISVLPKQYY TPLKKYHQIE DSVLLIIDSV SNAIRDQFIS   500
KLLFFAFAVS ISINVYLLNA AKIHTGYMNF QPQSNKIDDL VVQQKSATIE   550
FSETRSMPAS SGLETPVTAK DIIISEEIQN NECVYALSSQ DEPIRPLSNL   600
VELMEKEQLK NMNNTEVSNL VVNGKLPLYS LEKKLEDTTR AVLVRRKALS   650
TLAESPILVS EKLPFRNYDY DRVFGACCEN VIGYMPIPVG VIGPLIIDGT   700
SYHIPMATTE GCLVASAMRG CKAINAGGGA TTVLTKDGMT RGPVVRFPTL   750
IRSGACKIWL DSEEGQNSIK KAFNSTSRFA RLQHIQTCLA GDLLFMRFRT   800
TTGDAMGMNM ISKGVEYSLK QMVEEYGWED MEVVSVSGNY CTDKKPAAIN   850
WIEGRGKSVV AEATIPGDVV KSVLKSDVSA LVELNISKNL VGSAMAGSVG   900
GFNAHAANLV TALFLALGQD PAQNVESSNC ITLMKEVDGD LRISVSMPSI   950
EVGTIGGGTV LEPQGAMLDL LGVRGPHPTE PGANARQLAR IIACAVLAGE   1000
LSLCSALAAG HLVQSHMTHN RKTNKANELP QPSNKGPPCK TSALL        1045