SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHIS4
Systematic nameYCL030C
DescriptionHistidinol dehydrogenase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP00815
SGD YCL030C
Gene Ontology (GO)
Cellular componentGO:0005622intracellularTAS:SGD.
Molecular functionGO:0004399histidinol dehydrogenase activityTAS:SGD.
Molecular functionGO:0004635phosphoribosyl-AMP cyclohydrolase activityTAS:SGD.
Molecular functionGO:0004636phosphoribosyl-ATP diphosphatase activityTAS:SGD.
Biological processGO:0000105histidine biosynthetic processTAS:SGD.
EC number 3.5.4.19,3.6.1.31,1.1.1.23

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length799
Molecular weight87721

	---------+----------+----------+----------+----------+
MVLPILPLID DLASWNSKKE YVSLVGQVLL DGSSLSNEEI LQFSKEEEVP   50
LVALSLPSGK FSDDEIIAFL NNGVSSLFIA SQDAKTAEHL VEQLNVPKER   100
VVVEENGVFS NQFMVKQKFS QDKIVSIKKL SKDMLTKEVL GEVRTDRPDG   150
LYTTLVVDQY ERCLGLVYSS KKSIAKAIDL GRGVYYSRSR NEIWIKGETS   200
GNGQKLLQIS TDCDSDALKF IVEQENVGFC HLETMSCFGE FKHGLVGLES   250
LLKQRLQDAP EESYTRRLFN DSALLDAKIK EEAEELTEAK GKKELSWEAA   300
DLFYFALAKL VANDVSLKDV ENNLNMKHLK VTRRKGDAKP KFVGQPKAEE   350
EKLTGPIHLD VVKASDKVGV QKALSRPIQK TSEIMHLVNP IIENVRDKGN   400
SALLEYTEKF DGVKLSNPVL NAPFPEEYFE GLTEEMKEAL DLSIENVRKF   450
HAAQLPTETL EVETQPGVLC SRFPRPIEKV GLYIPGGTAI LPSTALMLGV   500
PAQVAQCKEI VFASPPRKSD GKVSPEVVYV AEKVGASKIV LAGGAQAVAA   550
MAYGTETIPK VDKILGPGNQ FVTAAKMYVQ NDTQALCSID MPAGPSEVLV   600
IADEDADVDF VASDLLSQAE HGIDSQVILV GVNLSEKKIQ EIQDAVHNQA   650
LQLPRVDIVR KCIAHSTIVL CDGYEEALEM SNQYAPEHLI LQIANANDYV   700
KLVDNAGSVF VGAYTPESCG DYSSGTNHTL PTYGYARQYS GANTATFQKF   750
ITAQNITPEG LENIGRAVMC VAKKEGLDGH RNAVKIRMSK LGLIPKDFQ    799