SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHIS1
Systematic nameYER055C
DescriptionATP phosphoribosyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP00498
SGD YER055C
Gene Ontology (GO)
Cellular componentGO:0005622intracellularTAS:SGD.
Molecular functionGO:0003879ATP phosphoribosyltransferase activityTAS:SGD.
Biological processGO:0000105histidine biosynthetic processTAS:SGD.
EC number 2.4.2.17

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length297
Molecular weight32266

	---------+----------+----------+----------+----------+
MDLVNHLTDR LLFAIPKKGR LYSKSVSILN GADITFHRSQ RLDIALSTSL   50
PVALVFLPAA DIPTFVGEGK CDLGITGVDQ VRESNVDVDL AIDLQFGNCK   100
LQVQVPVNGE YKKPEQLIGK TIVTSFVKLA EKYFADLEGT TVEKMTTRIK   150
FVSGSVEASC ALGIGDAIVD LVESGETMRA AGLVDIATVL STSAYLIESK   200
NPKSDKSLIA TIKSRIEGVM TAQRFVSCIY NAPEDKLPEL LKVTPGRRAP   250
TISKIDDEGW VAVSSMIERK TKGVVLDELK RLGASDIMVF EISNCRV      297