SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHEF3
Systematic nameYNL014W
AliasYEF3B, ZRG7
DescriptionElongation factor 3B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP53978
SGD YNL014W
Gene Ontology (GO)
Cellular componentGO:0005830cytosolic ribosome (sensu Eukaryota)IPI:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0003746translation elongation factor activityIGI:SGD.
Biological processGO:0006414translational elongationIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine (By similarity).

Sequence Information
Sequence length1044
Molecular weight115869

	---------+----------+----------+----------+----------+
MSDSQQSITV LEELFRKLET ATSETREGIS SELSSFLNGN IIEHDVPEVF   50
FDEFQKAIQS KQKALNTLGA VAYIANETNL SPSVEPYIVA TVPSVCSKAG   100
SKDNDVQLAA TKALKAIASA VNPVAVKALL PHLIHSLETS NKWKEKVAVL   150
EVISVLVDAA KEQIALRMPE LIPVLSESMW DTKKGVKEAA TTTITKATET   200
VDNKDIERFI PKLIECIANP NEVPETVHLL GATTFVAEVT PATLSIMVPL   250
LSRGLAERET SIKRKAAVII DNMCKLVEDP QVVAPFLGKL LPGLKNNFAT   300
IADPEAREVT LKALKTLRRV GNVGEDDVLP EISHAGDVST TLGVIKELLE   350
PEKVAPRFTI VVEYIAAIAA NLIDERIIDQ QTWFTHVTPY MTIFLHEKTA   400
KEILDDFRKR AVDNIPVGPN FQDEEDEGED LCNCEFSLAY GAKILLNKTQ   450
LRLKRGRRYG LCGPNGAGKS TLMRSIANGQ VDGFPTQDEC RTVYVEHDID   500
NTHSDMSVLD FVYSGNVGTK DVITSKLKEF GFSDEMIEMP IASLSGGWKM   550
KLALARAVLK DADILLLDEP TNHLDTVNVE WLVNYLNTCG ITSVIVSHDS   600
GFLDKVCQYI IHYEGLKLRK YKGNLSEFVQ KCPTAQSYYE LGASDLEFQF   650
PTPGYLEGVK TKQKAIVKVS NMTFQYPGTT KPQVSDVTFQ CSLSSRIAVI   700
GPNGAGKSTL INVLTGELLP TSGEVYTHEN CRIAYIKQHA FAHIESHLDK   750
TPSEYIQWRF QTGEDRETMD RANRQINEND AEAMNKIFKI EGTPRRVAGI   800
HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWL PRGELIESHS   850
KMVAEIDMKE ALASGQFRAL TRKEIELHCA MLGLDSELVS HSRIRGLSGG   900
QKVKLVLAAC TWQRPHLIVL DEPTNYLDRD SLGALSKALK AFEGGVIIIT   950
HSAEFTKNLT DEVWAVKDGK MTPSGHNWVA GQGAGPRIEK KEEEGDKFDA   1000
MGNKINSGKK KSKLSSAELR KKKKERMKKK KEMGDEYVSS DEDF         1044