SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameHAP4
Systematic nameYKL109W
DescriptionTranscriptional activator HAP4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14064
SGD YKL109W
Gene Ontology (GO)
Cellular componentGO:0016602CCAAT-binding factor complexTAS:SGD.
Molecular functionGO:0016563transcriptional activator activityTAS:SGD.
Biological processGO:0006109regulation of carbohydrate metabolic processTAS:SGD.
Biological processGO:0006350transcriptionTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length554
Molecular weight62412

	---------+----------+----------+----------+----------+
MTAKTFLLQA SASRPRSNHF KNEHNNIPLA PVPIAPNTNH HNNSSLEFEN   50
DGSKKKKKSS LVVRTSKHWV LPPRPRPGRR SSSHNTLPAN NTNNILNVGP   100
NSRNSSNNNN NNNIISNRKQ ASKEKRKIPR HIQTIDEKLI NDSNYLAFLK   150
FDDLENEKFH SSASSISSPS YSSPSFSSYR NRKKSEFMDD ESCTDVETIA   200
AHNSLLTKNH HIDSSSNVHA PPTKKSKLND FDLLSLSSTS SSATPVPQLT   250
KDLNMNLNFH KIPHKASFPD SPADFSPADS VSLIRNHSLP TNLQVKDKIE   300
DLNEIKFFND FEKLEFFNKY AKVNTNNDVN ENNDLWNSYL QSMDDTTGKN   350
SGNYQQVDND DNMSLLNLPI LEETVSSGQD DKVEPDEEDI WNYLPSSSSQ   400
QEDSSRALKK NTNSEKANIQ AKNDETYLFL QDQDESADSH HHDELGSEIT   450
LADNKFSYLP PTLEELMEEQ DCNNGRSFKN FMFSNDTGID GSAGTDDDYT   500
KVLKSKKIST SKSNANLYDL NDNNNDATAT NELDQSSFID DLDEDVDFLK   550
VQVF                                                     554