SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGYP1
Systematic nameYOR070C
DescriptionGTPase-activating protein GYP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ08484
SGD YOR070C
Gene Ontology (GO)
Cellular componentGO:0005794Golgi apparatusIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005097Rab GTPase activator activityIDA:SGD.
Biological processGO:0016192vesicle-mediated transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
87Phosphoserine.
91Phosphoserine.

Sequence Information
Sequence length637
Molecular weight73289

	---------+----------+----------+----------+----------+
MGVRSAAKEM HERDHNSDSS SLVTSLMKSW RISSASSSKK PSLYKMNTTE   50
STSLPSGYAS SADRDRRTSD GNFEAMAKQQ ASTRRTSNSY SPLRYVNPTL   100
STASNESPRP ALLLRQHHQR HHHHQQPRHS SSGSVGNNCS NSTEPNKKGD   150
RYFKDLDEDW SAVIDDYNMP IPILTNGGFG TPVAPTRTLS RKSTSSSINS   200
ISNMGTSAVR NSSSSFTYPQ LPQLQKEKTN DSKKTQLEIE NERDVQELNS   250
IIQRISKFDN ILKDKTIINQ QDLRQISWNG IPKIHRPVVW KLLIGYLPVN   300
TKRQEGFLQR KRKEYRDSLK HTFSDQHSRD IPTWHQIEID IPRTNPHIPL   350
YQFKSVQNSL QRILYLWAIR HPASGYVQGI NDLVTPFFET FLTEYLPPSQ   400
IDDVEIKDPS TYMVDEQITD LEADTFWCLT KLLEQITDNY IHGQPGILRQ   450
VKNLSQLVKR IDADLYNHFQ NEHVEFIQFA FRWMNCLLMR EFQMGTVIRM   500
WDTYLSETSQ EVTSSYSMSS NDIKPPVTPT EPRVASFVTP TKDFQSPTTA   550
LSNMTPNNAV EDSGKMRQSS LNEFHVFVCA AFLIKWSDQL MEMDFQETIT   600
FLQNPPTKDW TETDIEMLLS EAFIWQSLYK DATSHWL                 637