SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGVP36
Systematic nameYIL041W
DescriptionProtein GVP36

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K305;K313        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40531
SGD YIL041W
Gene Ontology (GO)
Cellular componentGO:0030173integral to Golgi membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
113Phosphothreonine.
114Phosphoserine.
319Phosphoserine.

Sequence Information
Sequence length326
Molecular weight36670

	---------+----------+----------+----------+----------+
MSFNAFASSL SKKLQEISTS VSEKTQELPS LAQSTQRMVQ ERLGQVTDIS   50
QLPREYTELE DKVDTIKLIY NHFLGVTAIY ENGSYDYPKY INESVNEFSR   100
SVASKLTELT HATSASEAQN ILVAPGPIKE PKTLNYALSK VALNSSECLN   150
KMFPTEEQPL ASALLQFSDV QAKIAQARIQ QDTLIQTKFN KNLRERLSFE   200
IGKADKCRKD VHSMRLRYDV ARTNLANNKK PEKEASLRVQ METLEDQFAQ   250
VTEDATVCLQ EVISHANFSE DLKELAKAQA EYFETSAGLM KEFLSNSFAE   300
EPEAKPEVAE EEKPQTAISM NDEDDA                             326

	* Ubiquitinated lysine is denoted as red color.