SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGUD1
Systematic nameYDL238C
DescriptionProbable guanine deaminase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ07729
SGD YDL238C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0008892guanine deaminase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number 3.5.4.3

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length489
Molecular weight55204

	---------+----------+----------+----------+----------+
MTKSDLLFDK FNDKHGKFLV FFGTFVDTPK LGELRIREKT SVGVLNGIIR   50
FVNRNSLDPV KDCLDHDSSL SPEDVTVVDI IGKDKTRNNS FYFPGFVDTH   100
NHVSQYPNVG VFGNSTLLDW LEKYTFPIEA ALANENIARE VYNKVISKTL   150
SHGTTTVAYY NTIDLKSTKL LAQLSSLLGQ RVLVGKVCMD TNGPEYYIED   200
TKTSFESTVK VVKYIRETIC DPLVNPIVTP RFAPSCSREL MQQLSKLVKD   250
ENIHVQTHLS ENKEEIQWVQ DLFPECESYT DVYDKYGLLT EKTVLAHCIH   300
LTDAEARVIK QRRCGISHCP ISNSSLTSGE CRVRWLLDQG IKVGLGTDVS   350
AGHSCSILTT GRQAFAVSRH LAMRETDHAK LSVSECLFLA TMGGAQVLRM   400
DETLGTFDVG KQFDAQMIDT NAPGSNVDMF HWQLKEKDQM QEQEQEQGQD   450
PYKNPPLLTN EDIIAKWFFN GDDRNTTKVW VAGQQVYQI               489