SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGSH1
Systematic nameYJL101C
DescriptionGlutamate--cysteine ligase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32477
SGD YJL101C
Gene Ontology (GO)
Cellular componentGO:0005622intracellularIDA:SGD.
Molecular functionGO:0004357glutamate-cysteine ligase activityIDA:SGD.
Biological processGO:0006750glutathione biosynthetic processIDA:SGD.
Biological processGO:0046686response to cadmium ionIDA:SGD.
Biological processGO:0009409response to coldIEP:SGD.
Biological processGO:0042493response to drugIMP:SGD.
Biological processGO:0009408response to heatIEP:SGD.
Biological processGO:0042542response to hydrogen peroxideIMP:SGD.
Biological processGO:0046689response to mercury ionIEP:SGD.
EC number 6.3.2.2

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length678
Molecular weight78254

	---------+----------+----------+----------+----------+
MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE   50
LEYMVVDFDD KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY   100
MLEATPASPY LNYVGSYVEV NMQKRRAIAE YKLSEYARQD SKNNLHVGSR   150
SVPLTLTVFP RMGCPDFINI KDPWNHKNAA SRSLFLPDEV INRHVRFPNL   200
TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE DKEAKLASKP   250
GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA   300
PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV   350
QDKVLEIPKS RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA   400
PLDYDLAKHF AHLYIRDPVS TFEELLNQDN KTSSNHFENI QSTNWQTLRF   450
KPPTQQATPD KKDSPGWRVE FRPFEVQLLD FENAAYSVLI YLIVDSILTF   500
SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND TDVETEDYSI   550
SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS   600
DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD   650
SKGELTSFLG AEIAEYVKKN KPSIESKC                           678