SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGSC2
Systematic nameYGR032W
AliasFKS2, GLS2
Description1,3-beta-glucan synthase component GSC2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K806        
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40989
SGD YGR032W
Gene Ontology (GO)
Cellular componentGO:00001481,3-beta-glucan synthase complexIDA:SGD.
Cellular componentGO:0030478actin capIDA:SGD.
Cellular componentGO:0005628prospore membraneIDA:SGD.
Molecular functionGO:00038431,3-beta-glucan synthase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:00060751,3-beta-glucan biosynthetic processIDA:SGD.
Biological processGO:0030476spore wall assembly (sensu Fungi)IGI:SGD.
EC number 2.4.1.34

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1895
Molecular weight216990

	---------+----------+----------+----------+----------+
MSYNDPNLNG QYYSNGDGTG DGNYPTYQVT QDQSAYDEYG QPIYTQNQLD   50
DGYYDPNEQY VDGTQFPQGQ DPSQDQGPYN NDASYYNQPP NMMNPSSQDG   100
ENFSDFSSYG PPSGTYPNDQ YTPSQMSYPD QDGSSGASTP YGNGVVNGNG   150
QYYDPNAIEM ALPNDPYPAW TADPQSPLPI EQIEDIFIDL TNKFGFQRDS   200
MRNMFDHFMT LLDSRSSRMS PEQALLSLHA DYIGGDTANY KKWYFAAQLD   250
MDDEIGFRNM KLGKLSRKAR KAKKKNKKAM QEASPEDTEE TLNQIEGDNS   300
LEAADFRWKS KMNQLSPFEM VRQIALFLLC WGEANQVRFT PECLCFIYKC   350
ASDYLDSAQC QQRPDPLPEG DFLNRVITPL YRFIRSQVYE IVDGRYVKSE   400
KDHNKVIGYD DVNQLFWYPE GIAKIVMEDG TRLIDLPAEE RYLKLGEIPW   450
DDVFFKTYKE TRSWLHLVTN FNRIWIMHIS VYWMYCAYNA PTFYTHNYQQ   500
LVDNQPLAAY KWATAALGGT VASLIQVAAT LCEWSFVPRK WAGAQHLSRR   550
FWFLCVIMGI NLGPVIFVFA YDKDTVYSTA AHVVGAVMFF VAVATLVFFS   600
VMPLGGLFTS YMKKSTRSYV ASQTFTASFA PLHGLDRWMS YLVWVTVFAA   650
KYAESYFFLI LSLRDPIRIL STTSMRCTGE YWWGNKICKV QPKIVLGLMI   700
ATDFILFFLD TYLWYIVVNT VFSVGKSFYL GISILTPWRN IFTRLPKRIY   750
SKILATTDME IKYKPKVLIS QIWNAIIISM YREHLLAIDH VQKLLYHQVP   800
SEIEGKRTLR APTFFVSQDD NNFETEFFPR DSEAERRISF FAQSLSTPIP   850
EPLPVDNMPT FTVLTPHYAE RILLSLREII REDDQFSRVT LLEYLKQLHP   900
VEWDCFVKDT KILAEETAAY ENNEDEPEKE DALKSQIDDL PFYCIGFKSA   950
APEYTLRTRI WASLRSQTLY RTISGFMNYS RAIKLLYRVE NPEIVQMFGG   1000
NADGLERELE KMARRKFKFL VSMQRLAKFK PHELENAEFL LRAYPDLQIA   1050
YLDEEPPLNE GEEPRIYSAL IDGHCEILEN GRRRPKFRVQ LSGNPILGDG   1100
KSDNQNHALI FYRGEYIQLI DANQDNYLEE CLKIRSVLAE FEELGIEQIH   1150
PYTPGLKYED QSTNHPVAIV GAREYIFSEN SGVLGDVAAG KEQTFGTLFA   1200
RTLAQIGGKL HYGHPDFINA TFMTTRGGVS KAQKGLHLNE DIYAGMNAVL   1250
RGGRIKHCEY YQCGKGRDLG FGTILNFTTK IGAGMGEQML SREYYYLGTQ   1300
LPIDRFLTFY YAHPGFHLNN LFIQLSLQMF MLTLVNLHAL AHESILCVYD   1350
RDKPITDVLY PIGCYNFHPA IDWVRRYTLS IFIVFWIAFV PIVVQELIER   1400
GLWKATQRFF RHILSLSPMF EVFAGQIYSS ALLSDIAVGG ARYISTGRGF   1450
ATSRIPFSIL YSRFAGSAIY MGSRSMLMLL FGTVAHWQAP LLWFWASLSA   1500
LIFAPFIFNP HQFAWEDFFL DYRDYIRWLS RGNNKYHRNS WIGYVRMSRS   1550
RVTGFKRKLV GDESEKSAGD ASRAHRTNLI MAEIIPCAIY AAGCFIAFTF   1600
INAQTGVKTT DEDRVNSTLR IIICTLAPIV IDIGVLFFCM GLSCCSGPLL   1650
GMCCKKTGSV MAGIAHGIAV VVHIVFFIVM WVLEGFSFVR MLIGVVTCIQ   1700
CQRLIFHCMT VLLLTREFKN DHANTAFWTG KWYSTGLGYM AWTQPTRELT   1750
AKVIELSEFA ADFVLGHVIL IFQLPVICIP KIDKFHSIML FWLKPSRQIR   1800
PPIYSLKQAR LRKRMVRRYC SLYFLVLIIF AGCIVGPAVA SAHVPKDLGS   1850
GLTGTFHNLV QPRNVSNNDT GSQMSTYKSH YYTHTPSLKT WSTIK        1895

	* Ubiquitinated lysine is denoted as red color.