SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGRR1
Systematic nameYJR090C
AliasCAT80, COT2
DescriptionSCF E3 ubiquitin ligase complex F-box protein GRR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2 AUTOCDC34RINGSCFGRR1  Proteasomal Degradation
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases."
Zhou P, Howley PM.
Mol Cell. 2(5):571-80 (1998) [9844630]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism."
Galan JM, Peter M.
Proc Natl Acad Sci U S A. 96(16):9124-9 (1999) [10430906]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP24814
SGD YJR090C
Gene Ontology (GO)
Cellular componentGO:0000142cellular bud neck contractile ringIDA:SGD.
Cellular componentGO:0000152nuclear ubiquitin ligase complexTAS:SGD.
Cellular componentGO:0019005SCF ubiquitin ligase complexIMP:SGD.
Molecular functionGO:0005515protein bindingTAS:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0000751cell cycle arrest in response to pheromoneIMP:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleTAS:SGD.
Biological processGO:0016567protein ubiquitinationIGI:SGD.
Biological processGO:0006974response to DNA damage stimulusIMP:SGD.
Biological processGO:0031146SCF-dependent proteasomal ubiquitin-depende...IPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
199Phosphoserine.

Sequence Information
Sequence length1151
Molecular weight132734

	---------+----------+----------+----------+----------+
MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS   50
TRPQMPSRTR ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL   100
NQNFSLTFQP QQQTNALNGI DINTVNTNLM NGVNVQIDQL NRLLPNLPEE   150
ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI LNEIELDNLK LKELRIDNSP   200
QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK SLYAYCHSKL   250
RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS   300
NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE   350
IIVKILYYRP HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD   400
YMHDTELNYF VGCKNLERLT LVFCKHITSV PISAVLRGCK FLQSVDITGI   450
RDVSDDVFDT LATYCPRVQG FYVPQARNVT FDSLRNFIVH SPMLKRIKIT   500
ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL TRLVQLREFR   550
ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK   600
LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC   650
TRIQYVDFAC CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL   700
RGRNDTLERV HLSYCSNLTI YPIYELLMSC PRLSHLSLTA VPSFLRPDIT   750
MYCRPAPSDF SENQRQIFCV FSGKGVHKLR HYLVNLTSPA FGPHVDVNDV   800
LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG LNQINGLNND   850
FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD   900
VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF   950
QFTGFLLHEM TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF   1000
IDKFIMVVQK YKLSTVVLRL YLKDNITLLT RQRELLIAHQ RSAWNNNNDN   1050
DANRNANNIV NIVSDAGAND TSNNETNNGN DDNETENPNF WRQFGNRMQI   1100
SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD EASGTPDEDM   1150
L                                                        1151