SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGPR1
Systematic nameYDL035C
DescriptionG protein-coupled receptor GPR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ12361
SGD YDL035C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0004930G-protein coupled receptor activityIMP:SGD.
Biological processGO:0007186G-protein coupled receptor protein signalin...IMP:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
Biological processGO:0001302replicative cell agingIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
369Phosphothreonine.
371Phosphothreonine.
373Phosphoserine.
903Phosphoserine.

Sequence Information
Sequence length961
Molecular weight110709

	---------+----------+----------+----------+----------+
MITEGFPPNL NALKGSSLLE KRVDSLRQLN TTTVNQLLGL PGMTSTFTAP   50
QLLQLRIIAI TASAVSLIAG CLGMFFLSKM DKRRKVFRHD LIAFLIICDF   100
LKAFILMIYP MIILINNSVY ATPAFFNTLG WFTAFAIEGA DMAIMIFAIH   150
FAILIFKPNW KWRNKRSGNM EGGLYKKRSY IWPITALVPA ILASLAFINY   200
NKLNDDSDTT IILDNNNYNF PDSPRQGGYK PWSAWCYLPP KPYWYKIVLS   250
WGPRYFIIIF IFAVYLSIYI FITSESKRIK AQIGDFNHNV LEEEKEKKKL   300
FGLGHWGKAK WYFRSYFKLP LLHLLRNLKN FFTISFIDPN EETDDSGSSN   350
GTFNFGESSN EIPTLFRKTN TGSDENVSAS GGVRLLDYNS AKPLDMSKYA   400
MSEQPDLERN NPFDCENDIT LNPSELVSKQ KEHKVTFSVE NEGLDTRKSS   450
MLGHQTFSCQ NSLESPLAMY DNKNDNSDIT SNIKEKGGII NNNSNNDDDD   500
NNNNNDNDND NNNSNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNSNNIKN   550
NVDNNNTNPA DNIPTLSNEA FTPSQQFSQE RVNNNADRCE NSSFTNVQQH   600
FQAQTYKQMK KRRAQIQKNL RAIFIYPLSY IGIWLFPIIA DALQYNHEIK   650
HGPTMWVTYI DTCVRPLSCL VDVIVYLFKE KPWNYSWAKT ESKYLIEKYI   700
LKGELGEKEI LKFCHSNWGK RGWYYRGKWK KRKCWKYSTN PLKRILWFVE   750
RFFKQLFELK LHFSFYDNCD DFEYWENYYS AKDSNDNKRT ESDETKTNSS   800
DRSLPSNSLE LQAMLNNITA EEVEVPLFWR IIHHIPMLGG IDLDELNRLL   850
KIRYNNDHFS LPGLKFALNQ NKSHDKHQDV STNSMVKSSF FSSNIVTNDD   900
ENSIEEDKNL RYSDASASEN YLVKPTIPGT TPDPIIEAQN DNDSSDSSGI   950
DLIAFLRNGP L                                             961