SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGPM1
Systematic nameYKL152C
AliasGPM
DescriptionPhosphoglycerate mutase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP00950
SGD YKL152C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004619phosphoglycerate mutase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006094gluconeogenesisIMP:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
EC number 5.4.2.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
12Phosphoserine.
116Phosphoserine.
127Phosphoserine.
128Phosphoserine.
185Phosphoserine.
197Phosphoserine.

Sequence Information
Sequence length247
Molecular weight27609

	---------+----------+----------+----------+----------+
MPKLVLVRHG QSEWNEKNLF TGWVDVKLSA KGQQEAARAG ELLKEKKVYP   50
DVLYTSKLSR AIQTANIALE KADRLWIPVN RSWRLNERHY GDLQGKDKAE   100
TLKKFGEEKF NTYRRSFDVP PPPIDASSPF SQKGDERYKY VDPNVLPETE   150
SLALVIDRLL PYWQDVIAKD LLSGKTVMIA AHGNSLRGLV KHLEGISDAD   200
IAKLNIPTGI PLVFELDENL KPSKPSYYLD PEAAAAGAAA VANQGKK      247