SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGPD2
Systematic nameYOL059W
AliasGPD3
DescriptionGlycerol-3-phosphate dehydrogenase [NAD+] 2, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP41911
SGD YOL059W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolTAS:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004367glycerol-3-phosphate dehydrogenase (NAD+) a...IMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006071glycerol metabolic processIMP:SGD.
Biological processGO:0006116NADH oxidationIMP:SGD.
EC number 1.1.1.8

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
70Phosphoserine.
72Phosphoserine.
75Phosphoserine.

Sequence Information
Sequence length440
Molecular weight49422

	---------+----------+----------+----------+----------+
MLAVRRLTRY TFLKRTHPVL YTRRAYKILP SRSTFLRRSL LQTQLHSKMT   50
AHTNIKQHKH CHEDHPIRRS DSAVSIVHLK RAPFKVTVIG SGNWGTTIAK   100
VIAENTELHS HIFEPEVRMW VFDEKIGDEN LTDIINTRHQ NVKYLPNIDL   150
PHNLVADPDL LHSIKGADIL VFNIPHQFLP NIVKQLQGHV APHVRAISCL   200
KGFELGSKGV QLLSSYVTDE LGIQCGALSG ANLAPEVAKE HWSETTVAYQ   250
LPKDYQGDGK DVDHKILKLL FHRPYFHVNV IDDVAGISIA GALKNVVALA   300
CGFVEGMGWG NNASAAIQRL GLGEIIKFGR MFFPESKVET YYQESAGVAD   350
LITTCSGGRN VKVATYMAKT GKSALEAEKE LLNGQSAQGI ITCREVHEWL   400
QTCELTQEFP LFEAVYQIVY NNVRMEDLPE MIEELDIDDE               440