SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGPA1
Systematic nameYHR005C
AliasCDC70, DAC1, SCG1
DescriptionGuanine nucleotide-binding protein alpha-1 subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP08539
SGD YHR005C
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIDA:SGD.
Cellular componentGO:0005834heterotrimeric G-protein complexIDA:SGD.
Molecular functionGO:0003924GTPase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000754adaptation to pheromone during conjugation ...IMP:SGD.
Biological processGO:0031684heterotrimeric G-protein complex cycleIMP:SGD.
Biological processGO:0048017inositol lipid-mediated signalingIMP:SGD.
Biological processGO:0000750pheromone-dependent signal transduction dur...IMP:SGD.
Biological processGO:0042493response to drugIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
189Phosphothreonine.
200Phosphoserine.

Sequence Information
Sequence length472
Molecular weight54076

	---------+----------+----------+----------+----------+
MGCTVSTQTI GDESDPFLQN KRANDVIEQS LQLEKQRDKN EIKLLLLGAG   50
ESGKSTVLKQ LKLLHQGGFS HQERLQYAQV IWADAIQSMK ILIIQARKLG   100
IQLDCDDPIN NKDLFACKRI LLKAKALDYI NASVAGGSDF LNDYVLKYSE   150
RYETRRRVQS TGRAKAAFDE DGNISNVKSD TDRDAETVTQ NEDADRNNSS   200
RINLQDICKD LNQEGDDQMF VRKTSREIQG QNRRNLIHED IAKAIKQLWN   250
NDKGIKQCFA RSNEFQLEGS AAYYFDNIEK FASPNYVCTD EDILKGRIKT   300
TGITETEFNI GSSKFKVLDA GGQRSERKKW IHCFEGITAV LFVLAMSEYD   350
QMLFEDERVN RMHESIMLFD TLLNSKWFKD TPFILFLNKI DLFEEKVKSM   400
PIRKYFPDYQ GRVGDAEAGL KYFEKIFLSL NKTNKPIYVK RTCATDTQTM   450
KFVLSAVTDL IIQQNLKKIG II                                 472