SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGNP1
Systematic nameYDR508C
DescriptionHigh-affinity glutamine permease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K34;K41;K132        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP48813
SGD YDR508C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0015359amino acid permease activityIDA:SGD.
Molecular functionGO:0005296L-proline permease activityIGI:SGD.
Biological processGO:0006865amino acid transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
123Phosphoserine.
124Phosphoserine.
127Phosphothreonine.

Sequence Information
Sequence length663
Molecular weight73598

	---------+----------+----------+----------+----------+
MTLGNRRHGR NNEGSSNMNM NRNDLDDVSH YEMKEIQPKE KQIGSIEPEN   50
EVEYFEKTVE KTIENMEYEG EHHASYLRRF IDSFRRAEGS HANSPDSSNS   100
NGTTPISTKD SSSQLDNELN RKSSYITVDG IKQSPQEQEQ KQENLKKSIK   150
PRHTVMMSLG TGIGTGLLVG NSKVLNNAGP GGLIIGYAIM GSCVYCIIQA   200
CGELAVIYSD LIGGFNTYPL FLVDPALGFS VAWLFCLQWL CVCPLELVTA   250
SMTIKYWTTS VNPDVFVVIF YVLIVVINVF GAKGYAEADF FFNCCKILMI   300
VGFFILAIII DCGGAGTDGY IGSKYWRDPG AFRGDTPIQR FKGVVATFVT   350
AAFAFGMSEQ LAMTASEQSN PRKAIPSAAK KMIYRILFVF LASLTLVGFL   400
VPYTSDQLLG AAGSATKASP YVIAVSSHGV RVVPHFINAV ILLSVLSVAN   450
GAFYTSSRIL MSLAKQGNAP KCFDYIDREG RPAAAMLVSA LFGVIAFCAS   500
SKKEEDVFTW LLAISGLSQL FTWITICLSH IRFRRAMKVQ GRSLGEVGYK   550
SQVGVWGSAY AVLMMVLALI AQFWVAIAPI GGGGKLSAQS FFENYLAMPI   600
WIALYIFYKV WKKDWSLFIP ADKVDLVSHR NIFDEELLKQ EDEEYKERLR   650
NGPYWKRVLD FWC                                           663

	* Ubiquitinated lysine is denoted as red color.