SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGLY1
Systematic nameYEL046C
DescriptionLow specificity L-threonine aldolase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K228        
2K228        
3         
4        Proteasomal Degradation
5K228        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[5]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP37303
SGD YEL046C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0004793threonine aldolase activityIDA:SGD.
Biological processGO:0006545glycine biosynthetic processIDA:SGD.
Biological processGO:0006567threonine catabolic processIDA:SGD.
EC number 4.1.2.5

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length387
Molecular weight42797

	---------+----------+----------+----------+----------+
MTEFELPPKY ITAANDLRSD TFTTPTAEMM EAALEASIGD AVYGEDVDTV   50
RLEQTVARMA GKEAGLFCVS GTLSNQIAIR THLMQPPYSI LCDYRAHVYT   100
HEAAGLAILS QAMVVPVVPS NGDYLTLEDI KSHYVPDDGD IHGAPTRLIS   150
LENTLHGIVY PLEELVRIKA WCMENGLKLH CDGARIWNAA AQSGVPLKQY   200
GEIFDSISIC LSKSMGAPIG SVLVGNLKFV KKATHFRKQQ GGGIRQSGMM   250
ARIALVNINN DWKSQLLYSH SLAHELAEYC EAKGIPLESP ADTNFVFINL   300
KAARMDPDVL VKKGLKYNVK LMGGRVSFHY QVTRDTLEKV KLAISEAFDY   350
AKEHPFDCNG PTQIYRSEST EVDVDGNAIR EIKTYKY                 387

	* Ubiquitinated lysine is denoted as red color.