SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameGLO1
Systematic nameYML004C
DescriptionLactoylglutathione lyase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP50107
SGD YML004C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004462lactoylglutathione lyase activityIGI:SGD.
Biological processGO:0006749glutathione metabolic processIGI:SGD.
Biological processGO:0019243methylglyoxal catabolic process to D-lactateIDA:SGD.
EC number 4.4.1.5

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length326
Molecular weight37209

	---------+----------+----------+----------+----------+
MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS   50
RKDFEEAKFS LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP   100
DYKINNGNEE PHRGFGHICF SVSDINKTCE ELESQGVKFK KRLSEGRQKD   150
IAFALGPDGY WIELITYSRE GQEYPKGSVG NKFNHTMIRI KNPTRSLEFY   200
QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV LELTHNWGTE   250
NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ   300
GRMKNIAFLK DPDGYSIEVV PHGLIA                             326