SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGLN3
Systematic nameYER040W
DescriptionNitrogen regulatory protein GLN3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP18494
SGD YER040W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Molecular functionGO:0016563transcriptional activator activityIDA:SGD.
Biological processGO:0045944positive regulation of transcription from R...IDA:SGD.
Biological processGO:0006808regulation of nitrogen utilizationIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
249Phosphoserine.
469Phosphoserine.
689Phosphoserine.

Sequence Information
Sequence length730
Molecular weight79383

	---------+----------+----------+----------+----------+
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA   50
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT   100
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS   150
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN   200
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD   250
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK   300
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI   350
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS   400
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN   450
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV   500
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS   550
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD   600
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR   650
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG   700
GKISEDNSTK GSSKESSAIA DELDWLKFGI                          730