SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGLN1
Systematic nameYPR035W
DescriptionGlutamine synthetase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K324        
2K324        
3         
4 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP32288
SGD YPR035W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004356glutamate-ammonia ligase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006807nitrogen compound metabolic processIEP:SGD.
EC number 6.3.1.2

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine.

Sequence Information
Sequence length370
Molecular weight41766

	---------+----------+----------+----------+----------+
MAEASIEKTQ ILQKYLELDQ RGRIIAEYVW IDGTGNLRSK GRTLKKRITS   50
IDQLPEWNFD GSSTNQAPGH DSDIYLKPVA YYPDPFRRGD NIVVLAACYN   100
NDGTPNKFNH RHEAAKLFAA HKDEEIWFGL EQEYTLFDMY DDVYGWPKGG   150
YPAPQGPYYC GVGAGKVYAR DMIEAHYRAC LYAGLEISGI NAEVMPSQWE   200
FQVGPCTGID MGDQLWMARY FLHRVAEEFG IKISFHPKPL KGDWNGAGCH   250
TNVSTKEMRQ PGGMKYIEQA IEKLSKRHAE HIKLYGSDND MRLTGRHETA   300
SMTAFSSGVA NRGSSIRIPR SVAKEGYGYF EDRRPASNID PYLVTGIMCE   350
TVCGAIDNAD MTKEFERESS                                     370

	* Ubiquitinated lysine is denoted as red color.