SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGIS1
Systematic nameYDR096W
DescriptionTranscriptional activator/repressor GIS1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03833
SGD YDR096W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0003677DNA bindingIDA:SGD.
Molecular functionGO:0051864histone demethylase activity (H3-K36 specific)IMP:SGD.
Molecular functionGO:0016566specific transcriptional repressor activityIDA:SGD.
Molecular functionGO:0016563transcriptional activator activityIDA:SGD.
Biological processGO:0016577histone demethylationIMP:SGD.
Biological processGO:0006644phospholipid metabolic processIMP:SGD.
Biological processGO:0030476spore wall assembly (sensu Fungi)IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
343Phosphoserine.
694Phosphoserine.
696Phosphoserine.

Sequence Information
Sequence length894
Molecular weight99481

	---------+----------+----------+----------+----------+
MEIKPVEVID GVPVFKPSMM EFANFQYFID EITKFGIENG IVKVIPPKEW   50
LELLEGSPPA ESLKTIQLDS PIQQQAKRWD KHENGVFSIE NEYDNKSYNL   100
TQWKNLAESL DSRISQGDFN DKTLKENCRV DSQQDCYDLA QLQILESDFW   150
KTIAFSKPFY AVDENSSIFP YDLTLWNLNN LPDSINSSNR RLLTGQSKCI   200
FPWHLDEQNK CSINYLHFGA PKQWYSIPSA NTDQFLKILS KEPSSNKENC   250
PAFIRHQNII TSPDFLRKNN IKFNRVVQFQ HEFIITFPYC MYSGFNYGYN   300
FGESIEFILD QQAVVRKQPL KCGCGNKKEE RKSGPFSNLS YDSNESEQRG   350
SITDNDNDLF QKVRSFDELL NHSSQELQNL EDNKNPLFSN INMNRPQSSS   400
LRSTTPNGVN QFLNMNQTTI SRISSPLLSR MMDLSNIVEP TLDDPGSKFK   450
RKVLTPQLPQ MNIPSNSSNF GTPSLTNTNS LLSNITATST NPSTTTNGSQ   500
NHNNVNANGI NTSAAASINN NISSTNNSAN NSSSNNNVST VPSSMMHSST   550
LNGTSGLGGD NDDNMLALSL ATLANSATAS PRLTLPPLSS PMNPNGHTSY   600
NGNMMNNNSG NGSNGSNSYS NGVTTAAATT TSAPHNLSIV SPNPTYSPNP   650
LSLYLTNSKN PLNSGLAPLS PSTSNIPFLK RNNVVTLNIS REASKSPISS   700
FVNDYRSPLG VSNPLMYSST INDYSNGTGI RQNSNNINPL DAGPSFSPLH   750
KKPKILNGND NSNLDSNNFD YSFTGNKQES NPSILNNNTN NNDNYRTSSM   800
NNNGNNYQAH SSKFGENEVI MSDHGKIYIC RECNRQFSSG HHLTRHKKSV   850
HSGEKPHSCP RCGKRFKRRD HVLQHLNKKI PCTQEMENTK LAES         894